Document Detail

A variant derived from rabbit aortic smooth muscle: phenotype modulation and restoration of smooth muscle characteristics in cells in culture.
MedLine Citation:
PMID:  2628418     Owner:  NLM     Status:  MEDLINE    
We examined the relationship between growth arrest of smooth muscle cells and structural changes in microfilament bundles, and also that between the structural changes and the actions of contractile agonist using a multipassagable variant cell line (SM-3) derived from rabbit aortic smooth muscle cells. The content of smooth muscle type alpha-actin increased with density-dependent growth arrest of the SM-3 cells, but was attenuated in the logarithmically growing cultures. As assessed cytochemically, the growth-arrested cells contained longitudinally oriented bundles of actin-containing microfilament and myosin-based filaments visualized with rhodamine-phalloidin and antibody against myosin light chain 20, respectively, whereas both actin- and myosin-containing structures in logarithmically growing cells showed slight, shortened, or diffused patterns. Electron microscopic examination of the growth-arrested cells revealed that the cells contained numerous and conspicuous microfilament bundles associated with many compact electron-dense bodies. In addition, pinocytotic vesicles were often found near the plasma membrane in the growth-arrested cells. SM-3 cells in the growth-arrested phase responded to prostaglandin F2 alpha (3-30 microM) and rat endothelin (0.1-1.0 microM) with a reversible contractile response, in association with monophosphorylation and/or diphosphorylation of the myosin light chain 20. However, the influence of the contractile agonists was greatly reduced during logarithmic growth. These results suggest that in the SM-3 cells in the growth-arrested phase, there is a restoration of the contractile architecture and the myosin light chain phosphorylation system. Thus, this SM-3 cell line is expected to serve as a useful model for examining biochemical and physiological phenomena of smooth muscle.
Y Sasaki; T Uchida; Y Sasaki
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biochemistry     Volume:  106     ISSN:  0021-924X     ISO Abbreviation:  J. Biochem.     Publication Date:  1989 Dec 
Date Detail:
Created Date:  1990-04-17     Completed Date:  1990-04-17     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0376600     Medline TA:  J Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  1009-18     Citation Subset:  IM    
Life Science Center, Biochemical Research Lab., Asahi Chemical Industry, Miyazaki.
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MeSH Terms
Actins / biosynthesis
Aorta / cytology
Cell Division
Cells, Cultured
Culture Media
Cytoskeleton / metabolism
Dinoprost / pharmacology
Genetic Variation
Muscle Contraction / physiology
Muscle, Smooth, Vascular / cytology*
Reg. No./Substance:
0/Actins; 0/Culture Media; 551-11-1/Dinoprost

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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