Document Detail


A variant derived from rabbit aortic smooth muscle: phenotype modulation and restoration of smooth muscle characteristics in cells in culture.
MedLine Citation:
PMID:  2628418     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We examined the relationship between growth arrest of smooth muscle cells and structural changes in microfilament bundles, and also that between the structural changes and the actions of contractile agonist using a multipassagable variant cell line (SM-3) derived from rabbit aortic smooth muscle cells. The content of smooth muscle type alpha-actin increased with density-dependent growth arrest of the SM-3 cells, but was attenuated in the logarithmically growing cultures. As assessed cytochemically, the growth-arrested cells contained longitudinally oriented bundles of actin-containing microfilament and myosin-based filaments visualized with rhodamine-phalloidin and antibody against myosin light chain 20, respectively, whereas both actin- and myosin-containing structures in logarithmically growing cells showed slight, shortened, or diffused patterns. Electron microscopic examination of the growth-arrested cells revealed that the cells contained numerous and conspicuous microfilament bundles associated with many compact electron-dense bodies. In addition, pinocytotic vesicles were often found near the plasma membrane in the growth-arrested cells. SM-3 cells in the growth-arrested phase responded to prostaglandin F2 alpha (3-30 microM) and rat endothelin (0.1-1.0 microM) with a reversible contractile response, in association with monophosphorylation and/or diphosphorylation of the myosin light chain 20. However, the influence of the contractile agonists was greatly reduced during logarithmic growth. These results suggest that in the SM-3 cells in the growth-arrested phase, there is a restoration of the contractile architecture and the myosin light chain phosphorylation system. Thus, this SM-3 cell line is expected to serve as a useful model for examining biochemical and physiological phenomena of smooth muscle.
Authors:
Y Sasaki; T Uchida; Y Sasaki
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biochemistry     Volume:  106     ISSN:  0021-924X     ISO Abbreviation:  J. Biochem.     Publication Date:  1989 Dec 
Date Detail:
Created Date:  1990-04-17     Completed Date:  1990-04-17     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0376600     Medline TA:  J Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  1009-18     Citation Subset:  IM    
Affiliation:
Life Science Center, Biochemical Research Lab., Asahi Chemical Industry, Miyazaki.
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MeSH Terms
Descriptor/Qualifier:
Actins / biosynthesis
Animals
Aorta / cytology
Cell Division
Cells, Cultured
Culture Media
Cytoskeleton / metabolism
Dinoprost / pharmacology
Genetic Variation
Histocytochemistry
Muscle Contraction / physiology
Muscle, Smooth, Vascular / cytology*
Phenotype
Rabbits
Chemical
Reg. No./Substance:
0/Actins; 0/Culture Media; 551-11-1/Dinoprost

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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