Document Detail


The variable expression of lectin-like oxidized low-density lipoprotein receptor (LOX-1) and signs of autophagy and apoptosis in freshly harvested human granulosa cells depend on gonadotropin dose, age, and body weight.
MedLine Citation:
PMID:  19342029     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To extend our recent observations on lectin-like oxidized low-density lipoprotein receptor (LOX-1) expression in human granulosa cell cultures with freshly harvested granulosa cells. DESIGN: Clinical research. SETTING: Institute of Anatomy and Clinic for Reproductive Medicine. PATIENT(S): Women undergoing IVF therapy were classified by total FSH dose, age, and body mass index. MAIN OUTCOME MEASURE(S): Purified granulosa cells were studied by Western blot and morphology for the presence of LOX-1, microtubule-associated light-chain protein 3 (LC3) and autophagosomes, which are both autophagic markers, cleaved caspase-3 for apoptosis, and apoptosis-inducing factor (AIF) for caspase-independent apoptosis. INTERVENTION(S): None. RESULTS: Active LOX-1 was found in all samples, being at its maximum in the younger obese group with a total FSH dose <2,000 IU. The LC3 II/LC3 I ratio, indicative of reparative autophagy, was at its maximum in younger normal-weight patients and increased under total FSH dose >2,000 IU. Autophagosomes in ultrathin sections were indicative of reparative autophagy. Cleaved caspase-3 was absent in all groups. The apoptotic AIF form was up-regulated in older patients. Unpurified granulosa cells consisted of approximately 20% dead cells in the younger normal-weight group compared with up to 50% in the older obese group. CONCLUSION(S): The regulation of LOX-1 and of cell death in granulosa cells depends on oxidative stress. It becomes excessive during aging and obesity, because the power of reparative autophagy fades and antioxidant efficiency declines.
Authors:
Constanze Vilser; Heike Hueller; Marcin Nowicki; Fayez A Hmeidan; Verona Blumenauer; Katharina Spanel-Borowski
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-04-01
Journal Detail:
Title:  Fertility and sterility     Volume:  93     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-17     Completed Date:  2010-06-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2706-15     Citation Subset:  IM    
Copyright Information:
Copyright 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
Affiliation:
Institute of Anatomy, University of Leipzig, Leipzig, Germany.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aging
Apoptosis / physiology*
Apoptosis Inducing Factor / biosynthesis
Autophagy / physiology*
Body Mass Index
Caspase 3 / biosynthesis
Female
Granulosa Cells / metabolism*
Humans
Microtubule-Associated Proteins / biosynthesis
Obesity / metabolism
Scavenger Receptors, Class E / biosynthesis*
Chemical
Reg. No./Substance:
0/Apoptosis Inducing Factor; 0/Microtubule-Associated Proteins; 0/OLR1 protein, human; 0/Scavenger Receptors, Class E; 0/light chain 3, human; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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