Document Detail

The variable expression of lectin-like oxidized low-density lipoprotein receptor (LOX-1) and signs of autophagy and apoptosis in freshly harvested human granulosa cells depend on gonadotropin dose, age, and body weight.
MedLine Citation:
PMID:  19342029     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To extend our recent observations on lectin-like oxidized low-density lipoprotein receptor (LOX-1) expression in human granulosa cell cultures with freshly harvested granulosa cells. DESIGN: Clinical research. SETTING: Institute of Anatomy and Clinic for Reproductive Medicine. PATIENT(S): Women undergoing IVF therapy were classified by total FSH dose, age, and body mass index. MAIN OUTCOME MEASURE(S): Purified granulosa cells were studied by Western blot and morphology for the presence of LOX-1, microtubule-associated light-chain protein 3 (LC3) and autophagosomes, which are both autophagic markers, cleaved caspase-3 for apoptosis, and apoptosis-inducing factor (AIF) for caspase-independent apoptosis. INTERVENTION(S): None. RESULTS: Active LOX-1 was found in all samples, being at its maximum in the younger obese group with a total FSH dose <2,000 IU. The LC3 II/LC3 I ratio, indicative of reparative autophagy, was at its maximum in younger normal-weight patients and increased under total FSH dose >2,000 IU. Autophagosomes in ultrathin sections were indicative of reparative autophagy. Cleaved caspase-3 was absent in all groups. The apoptotic AIF form was up-regulated in older patients. Unpurified granulosa cells consisted of approximately 20% dead cells in the younger normal-weight group compared with up to 50% in the older obese group. CONCLUSION(S): The regulation of LOX-1 and of cell death in granulosa cells depends on oxidative stress. It becomes excessive during aging and obesity, because the power of reparative autophagy fades and antioxidant efficiency declines.
Constanze Vilser; Heike Hueller; Marcin Nowicki; Fayez A Hmeidan; Verona Blumenauer; Katharina Spanel-Borowski
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-04-01
Journal Detail:
Title:  Fertility and sterility     Volume:  93     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-17     Completed Date:  2010-06-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2706-15     Citation Subset:  IM    
Copyright Information:
Copyright 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
Institute of Anatomy, University of Leipzig, Leipzig, Germany.
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MeSH Terms
Apoptosis / physiology*
Apoptosis Inducing Factor / biosynthesis
Autophagy / physiology*
Body Mass Index
Caspase 3 / biosynthesis
Granulosa Cells / metabolism*
Microtubule-Associated Proteins / biosynthesis
Obesity / metabolism
Scavenger Receptors, Class E / biosynthesis*
Reg. No./Substance:
0/Apoptosis Inducing Factor; 0/Microtubule-Associated Proteins; 0/OLR1 protein, human; 0/Scavenger Receptors, Class E; 0/light chain 3, human; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3

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