Document Detail

The value of molecular studies for the diagnosis of congenital Chagas disease in northeastern Argentina.
MedLine Citation:
PMID:  18385359     Owner:  NLM     Status:  MEDLINE    
Polymerase chain reaction (PCR) as a diagnostic tool for congenital Chagas disease is scarcely used. To assess the usefulness of PCR, a study in neonates was carried out to compare PCR with standard methods; that is bloodstream parasites by microhematocrit, and/or seroconversion by two conventional procedures. Seventeen neonates, born to mothers with positive Trypanosoma cruzi serology were analyzed by serologic, parasitological and molecular procedures, in blood samples taken at birth, and when aged 2-4 and 9 months. For a better comparison of sensitivity between both parasitological methods (microhematocrit and PCR), the number of samples of neonates studied at birth extended to 121. In children followed-up until 9 months of age, a 100% correlation between PCR and routine diagnosis was found, with PCR showing its highest sensitivity from Month 2 onwards. Comparative analysis between both parasitological methods, on samples taken at birth, showed a higher sensitivity of PCR respect the microhematocrit (P < 0.0008).
Cristina N Diez; Silvia Manattini; Juan Carlos Zanuttini; Oscar Bottasso; Iván Marcipar
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of tropical medicine and hygiene     Volume:  78     ISSN:  1476-1645     ISO Abbreviation:  Am. J. Trop. Med. Hyg.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-04-03     Completed Date:  2008-08-01     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0370507     Medline TA:  Am J Trop Med Hyg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  624-7     Citation Subset:  AIM; IM    
Laboratorio de Tecnología Inmunológica, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina.
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MeSH Terms
Argentina / epidemiology
Chagas Disease / blood,  congenital*,  diagnosis*,  epidemiology
Infant, Newborn
Infectious Disease Transmission, Vertical
Polymerase Chain Reaction / methods
Pregnancy Complications, Infectious / parasitology*
Serologic Tests
Trypanosoma cruzi / genetics*,  isolation & purification

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