Document Detail


The value of calcofluor white in the diagnosis of mycotic and Acanthamoeba infections of the eye and ocular adnexa.
MedLine Citation:
PMID:  2436125     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In search of an easy and quick method for the diagnosis of fungal and Acanthamoeba infections of the eye, the authors have used calcofluor white (CFW), a fluorescent brightener with marked affinity for chitin and cellulose which are present in the cell walls of fungi and cysts of Acanthamoeba. Paraffin-embedded tissues containing mycotic organisms were stained with CFW and examined under a fluorescent microscope. Several fungi such as Candida sp, Aspergillus sp, Phycomycetes, Fusarium solani, Histoplasma capsulatum, Cryptococcus neoformans, Rhinosporidium seeberi, and others were studied. Trephine corneal biopsy specimens (2 mm) and methanol-fixed corneal scrapings containing Acanthamoeba sp were also examined. The walls of fungi and Acanthamoeba cysts stained bright greenish white in contrast to the reddish-orange background of the supporting tissues. The results demonstrate that the CFW method is a highly reliable and rapid technique for identification of mycotic organisms as well as cysts of Acanthamoeba. This method may also be applied in surgical pathology when performing frozen sections, as well as cytologic examination of corneal smears, vitrectomy specimens, and others.
Authors:
H M Marines; M S Osato; R L Font
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Ophthalmology     Volume:  94     ISSN:  0161-6420     ISO Abbreviation:  Ophthalmology     Publication Date:  1987 Jan 
Date Detail:
Created Date:  1987-04-27     Completed Date:  1987-04-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7802443     Medline TA:  Ophthalmology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  23-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amebiasis / diagnosis*,  parasitology,  pathology
Benzenesulfonates / diagnostic use*
Eye / microbiology,  parasitology
Eye Diseases / diagnosis,  microbiology,  parasitology
Humans
Microscopy, Fluorescence
Mycoses / diagnosis*,  microbiology,  pathology
Orbit / microbiology,  parasitology
Orbital Diseases / diagnosis,  microbiology,  parasitology
Staining and Labeling
Chemical
Reg. No./Substance:
0/Benzenesulfonates; 4404-43-7/C.I. Fluorescent Brightening Agent 28

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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