Document Detail


The value of the Lugol's iodine staining technique for the identification of vaginal epithelial cells.
MedLine Citation:
PMID:  7524629     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This paper reports on the specificity of the Lugol's iodine staining technique for the detection of vaginal epithelial cells on penile swabs. Air-dried swabs taken from the glans of the penis of 153 hospital patients and from 50 healthy volunteers, whose last sexual intercourse had taken place at least 5 days previously, were stained with Lugol's solution. Glycogenated cells were found in more than 50% of the cases studied, even in healthy volunteers without urethritis. In almost all of these cases the smear contained at least a few polygonal nucleated epithelial cells showing an unequivocal positive Lugol reaction. These cells cannot be distinguished from superficial or intermediate vaginal cells, by cytomorphology or staining. Urinary tract infections had no influence on the glycogen content of male squamous epithelial cells. On the basis of these results the Lugol's method can no longer be assumed to prove the presence of vaginal cells in penile swabs.
Authors:
R Hausmann; C Pregler; B Schellmann
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Publication Detail:
Type:  Clinical Trial; Controlled Clinical Trial; Journal Article    
Journal Detail:
Title:  International journal of legal medicine     Volume:  106     ISSN:  0937-9827     ISO Abbreviation:  Int. J. Legal Med.     Publication Date:  1994  
Date Detail:
Created Date:  1994-12-20     Completed Date:  1994-12-20     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9101456     Medline TA:  Int J Legal Med     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  298-301     Citation Subset:  IM    
Affiliation:
Institut für Rechtsmedizin, Friedrich-Alexander-Universität Erlangen-Nürnberg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aged, 80 and over
Bias (Epidemiology)
Coitus
Epithelium / chemistry
Female
Forensic Medicine / methods
Glycogen / analysis
Humans
Iodides*
Male
Middle Aged
Penis / cytology*
Sensitivity and Specificity
Staining and Labeling*
Time Factors
Urinary Tract Infections / pathology
Vagina / cytology*
Chemical
Reg. No./Substance:
0/Iodides; 12298-68-9/Lugol's solution; 9005-79-2/Glycogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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