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A validated LC-MS/MS method for rapid determination of brazilin in rat plasma and its application to a pharmacokinetic study.
MedLine Citation:
PMID:  23303567     Owner:  NLM     Status:  Publisher    
Brazilin is a major homoisoflavonoid component isolated from the dried heartwood of traditional Chinese medicine Caesalpinia sappan L., which is a natural red pigment used for histological staining. Herein a sensitive, specific and rapid analytical LC-MS/MS method was established and validated for brazilin in rat plasma. After a simple step of protein precipitation using acetonitrile, plasma samples were analyzed using an LC-MS/MS system. Brazilin and the IS (protosappanin B) were separated on a Diamonsil C(18) analytical column (150 × 4.6 mm, 5 µm) using a mixture of water and 10 m m ammonium acetate in methanol (20:80, v/v) as mobile phase at a flow rate of 0.6 mL/min. The method was sensitive with a lower limit of quantitation of 10.0 ng/mL, with good linearity (r(2)  ≥ 0.99) over the linear range 10.0-5000 ng/mL. All the validation data, such as accuracy and precision, matrix effect, extraction recovery and stability tests were within the required limits. The assay method was successfully applied to evaluate the pharmacokinetics parameters of brazilin after an oral dose of 100 mg/kg brazilin in rats. Copyright © 2013 John Wiley & Sons, Ltd.
Zhipeng Deng; Xin Wang; Huanxin Zhao; Shuxiang Cui; Qingqiang Yao; Hong Bai
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-10
Journal Detail:
Title:  Biomedical chromatography : BMC     Volume:  -     ISSN:  1099-0801     ISO Abbreviation:  Biomed. Chromatogr.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8610241     Medline TA:  Biomed Chromatogr     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 John Wiley & Sons, Ltd.
Institute of Materia Medica, Shandong Academy of Medical Sciences, No. 18877 Jingshi Road, Jinan, 250062, People's Republic of China.
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