Document Detail


vMAlteration of bile acid metabolism in pseudo germ-free rats.
MedLine Citation:
PMID:  23212639     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
To characterize the impact of gut microbiota on host bile acid metabolism, we investigated the metabolic profiles of oxysterols and bile acids (BAs) in a conventional rat model (SD) (n=5) and its pseudo germ-free (GF) equivalent (n=5). GF rats were developed by the oral administration of bacitracin, neomycin and streptomycin (200 mg/kg, each) twice a day for 6 days. Urinary levels of oxysterols and bile acid metabolites were quantified using gas chromatography-mass spectrometry (GC-MS). The activity levels of enzymes involved in the bile acid metabolic pathway were determined through urinary concentration ratio between product to precursor. Cholic acid (CA) and α-/β-muricholic acid (α-/β-MCA) were significantly elevated at pseudo germ-free condition. An increase of hydroxylase (cholesterol 7α-hydroxylase, oxysterol 7α-hydroxylase and cytochrome P450 scc) and a significant decrease of 7α-dehydroxylase were observed. The urinary concentration ratio of primary bile acids, a marker for hepatotoxicity, increased in pseudo germfree conditions. Therefore, it was found that gut microbiota could play a significant role in the bile acids homeostasis and metabolism.
Authors:
Salil Kumar Bhowmik; Ji Hye An; Soo Hyun Lee; Byung Hwa Jung
Related Documents :
12855719 - Phospholipid composition of several clinically relevant corynebacterium species as dete...
25061589 - Omega-3 polyunsaturated fatty acid for cholestasis due to bile duct paucity.
17250549 - The differing embryonic origins of retinal and uveal (iris/ciliary body and choroid) me...
16666199 - Effects of ozone and peroxyacetyl nitrate on polar lipids and fatty acids in leaves of ...
3922279 - Changes in fatty acid composition of the cardiac phospholipids of the cotton-eared marm...
5411429 - Metabolism of endogenous sterol ester by the superovulated rat ovary in vitro.
6115549 - Slow-reacting substances and their formation by a lipoxygenase pathway.
8814309 - Two phosphatidylethanol classes separated by thin layer chromatography are produced by ...
12657299 - Tyrolobibenzyls e and f from scorzonera humilis and distribution of caffeic acid deriva...
Publication Detail:
Type:  Journal Article     Date:  2012-12-04
Journal Detail:
Title:  Archives of pharmacal research     Volume:  35     ISSN:  0253-6269     ISO Abbreviation:  Arch. Pharm. Res.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-12-05     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8000036     Medline TA:  Arch Pharm Res     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  1969-77     Citation Subset:  IM    
Affiliation:
Molecular Recognition Research Center, Korea Institute of Science and Technology, Seoul, 136-791, Korea.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Enhanced IL-12p40 production in LPS-stimulated macrophages by inhibiting JNK activation by artemisin...
Next Document:  Peptidomimetic small-molecule compounds promoting cardiogenesis of stem cells.