| Heparin/heparan sulfate/CD44-v3 enhances cell migration in term placenta-derived immortalized human trophoblast cells. | |
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MedLine Citation:
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PMID: 22321833 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The function of CD44-v3 and heparin/heparan sulfate (HS) signaling was investigated during trophoblast cell migration to identify their role in the renewal of syncytial layer damage caused by increased hemodynamic turbulence in the intervillous space and maintenance of syncytial integrity in pre-eclampsia. We evaluated the effect of heparin/HS/CD44-v3-mediated processes during scratch wound closure in monolayer immortalized human trophoblast cells derived from term placenta (TCL-1 cells). Western blot analysis showed that these cultured human trophoblast cells express the epidermal growth factor receptor and CD44-v3 but do not express syndecan 4. An in vitro scratch wound healing assay showed enhanced migration of trophoblast cells in a dose-dependent manner in the presence of heparin compared with controls when cultured under serum-free conditions. Conversely, an anti-CD44 function-blocking antibody and CD44 siRNA suppressed the migration of trophoblast cells in the presence of heparin in a similar scratch assay. Furthermore, both heparin treatment and in vitro scratch wounding induced the phosphorylation of p21-activated kinase 1 (PAK1), whereas the anti-CD44-v3 antibody suppressed the heparin-induced phosphorylation of PAK1 in trophoblast cells. These results indicate that heparin/HS/CD44-v3-mediated signaling, in the absence of growth factor networks, enhances the direct repair of the damaged trophoblast layer through the migration of trophoblast cells. This renewed cell coverage may lead to the maintenance of syncytiotrophoblast cell function and an associated reduction in pathogenic soluble factors derived from the damaged trophoblast cells. |
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Authors:
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Naoko Suga; Motoi Sugimura; Taro Koshiishi; Takashi Yorifuji; Shintaro Makino; Satoru Takeda |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-05-03 |
Journal Detail:
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Title: Biology of reproduction Volume: 86 ISSN: 1529-7268 ISO Abbreviation: Biol. Reprod. Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-05-04 Completed Date: 2012-09-17 Revised Date: 2012-12-06 |
Medline Journal Info:
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Nlm Unique ID: 0207224 Medline TA: Biol Reprod Country: United States |
Other Details:
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Languages: eng Pagination: 134, 1-8 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynecology, School of Medicine, Juntendo University, Tokyo, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antibodies, Blocking
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metabolism Anticoagulants / pharmacology* Antigens, CD44 / biosynthesis*, genetics, immunology Cell Movement / drug effects* Cells, Cultured Female Heparin / pharmacology* Heparitin Sulfate / pharmacology* Humans Phosphorylation Pregnancy RNA, Small Interfering / administration & dosage Receptor, Epidermal Growth Factor / biosynthesis Signal Transduction / drug effects Syndecan-4 / biosynthesis Trophoblasts / drug effects*, metabolism* p21-Activated Kinases / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Blocking; 0/Anticoagulants; 0/Antigens, CD44; 0/CD44 protein, human; 0/RNA, Small Interfering; 0/SDC4 protein, human; 0/Syndecan-4; 9005-49-6/Heparin; 9050-30-0/Heparitin Sulfate; EC 2.7.10.1/EGFR protein, human; EC 2.7.10.1/Receptor, Epidermal Growth Factor; EC 2.7.11.1/PAK1 protein, human; EC 2.7.11.1/p21-Activated Kinases |
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