Document Detail

v-raf suppresses apoptosis and promotes growth of interleukin-3-dependent myeloid cells.
MedLine Citation:
PMID:  8036007     Owner:  NLM     Status:  MEDLINE    
Interleukin-3 (IL-3) is required for the proliferation, survival and differentiation of myeloid progenitors. In the absence of IL-3, murine myeloid 32D.3 cells accumulate in the G1 phase of the cell cycle and subsequently undergo programmed cell death, or apoptosis. Here we demonstrate that enforced expression of the v-raf oncogene suppresses apoptosis of myeloid 32D.3 cells following the withdrawal of IL-3. Surprisingly, steady state levels of Bcl-2, an oncogene known to suppress apoptosis, were not dependent upon IL-3 in 32D.3 cells and its levels were not augmented in v-raf clones. This suggests that ability of v-raf to suppress apoptosis in the absence of ligand is either Bcl-2 independent or that v-raf kinase promotes Bcl-2 function. v-raf also promoted growth of these cells in the presence of IL-3. v-raf clones proliferated at an increased rate due to a shortened G1 phase and had decreased requirements for IL-3 for growth. Therefore, transformation of myeloid cells by v-raf involves signaling pathways which promote both cell cycle progression and cell survival.
J L Cleveland; J Troppmair; G Packham; D S Askew; P Lloyd; M González-Garcia; G Nuñez; J N Ihle; U R Rapp
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Oncogene     Volume:  9     ISSN:  0950-9232     ISO Abbreviation:  Oncogene     Publication Date:  1994 Aug 
Date Detail:
Created Date:  1994-08-15     Completed Date:  1994-08-15     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8711562     Medline TA:  Oncogene     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2217-26     Citation Subset:  IM    
Department of Biochemistry, St. Jude Children's Research Hospital, Memphis, Tennessee 38105.
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MeSH Terms
Bone Marrow Cells*
Cell Division
Cells, Cultured
Genes, myc
Interleukin-3 / physiology*
Oncogene Proteins v-raf
Proto-Oncogene Proteins / physiology
Proto-Oncogene Proteins c-bcl-2
Retroviridae Proteins, Oncogenic / genetics,  physiology*
Signal Transduction
Grant Support
Reg. No./Substance:
0/Interleukin-3; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/Retroviridae Proteins, Oncogenic; EC Proteins v-raf

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