Document Detail


The utilization of [3H] sugars by non-malignant and malignant human breast.
MedLine Citation:
PMID:  3746524     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The utilization of [3H] sugars and leucine by non-malignant and malignant human breast has been assessed using an organ culture technique with subsequent tissue autoradiography. The uptake of sugars by normal and hyperplastic breast was generally constant, with some differences observed in the utilization of galactose by acini of normal and hyperplastic tissues. After 24 h incubation localization was predominantly at the luminal cell periphery. The utilization of sugars by carcinomas was much more variable. Differences were observed between adjacent cells and cell groups of the same tumour. The uptake of individual sugars within a carcinoma was also varied being either similar to, or greater or lesser than normal breast. Variation between carcinomas was also present. No correlation between type and differentiation was noted in this respect, but there was between localization of sugars and differentiation. Better differentiated areas in tumours showed patterns similar to non-malignant breast whilst localization in poorly differentiated cell groups was cytoplasmic. The uptake of leucine was more constant and proved to be a useful indicator of viability. While this approach cannot give information with regard to differences in glycoprotein structure between non-malignant and malignant breast, it has been of value in determining the heterogeneity of tumour cells with regard to the enzymes involved in glycosylation. As such it would be of use in assessing the uniformity of response to agents modifying glycosylation.
Authors:
R A Walker; P R Sanderson; S J Day
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of pathology     Volume:  149     ISSN:  0022-3417     ISO Abbreviation:  J. Pathol.     Publication Date:  1986 Jul 
Date Detail:
Created Date:  1986-10-16     Completed Date:  1986-10-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0204634     Medline TA:  J Pathol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  173-81     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Breast / metabolism*
Breast Diseases / metabolism
Breast Neoplasms / metabolism*
Female
Fucose / metabolism*
Galactose / metabolism*
Glucosamine / metabolism*
Humans
Hyperplasia / metabolism
Leucine / metabolism
Tritium
Chemical
Reg. No./Substance:
10028-17-8/Tritium; 26566-61-0/Galactose; 3416-24-8/Glucosamine; 3713-31-3/Fucose; 61-90-5/Leucine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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