Document Detail

The utility of restriction endonuclease analysis and phage typing in the epidemiologic investigation of a Staphylococcus aureus outbreak in a neonatal nursery.
MedLine Citation:
PMID:  1319299     Owner:  NLM     Status:  MEDLINE    
Outbreaks of Staphylococcus aureus infections in neonatal units require prompt investigation and implementation of control measures. From January to March 1990, a marked increase in the number of S. aureus infections was observed in a neonatal nursery. Twenty-seven S. aureus isolates from 23 patients were analyzed by phage typing and restriction endonuclease analysis (REA). Only nine strains were differentiated by phage type. However, REA with HindIII, CfoI, and ClaI differentiated 20 strains. The REA results indicated that the outbreak was due to several different S. aureus strains and did not represent transmission of a single epidemic strain. REA may enable more accurate determination of the presence or absence of an epidemic strain during an outbreak than would traditional methods such as phage typing.
D H Pekkala; D E Low; P A Wyper; D Rose; D Sturman; M F Pritchard; L Panaro; A E Simor
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Diagnostic microbiology and infectious disease     Volume:  15     ISSN:  0732-8893     ISO Abbreviation:  Diagn. Microbiol. Infect. Dis.     Publication Date:    1992 May-Jun
Date Detail:
Created Date:  1992-07-29     Completed Date:  1992-07-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8305899     Medline TA:  Diagn Microbiol Infect Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  307-11     Citation Subset:  IM    
Department of Microbiology, Mount Sinai Hospital, Toronto, Ontario, Canada.
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MeSH Terms
Bacteriophage Typing*
Cross Infection / epidemiology,  microbiology*
DNA Restriction Enzymes*
DNA, Bacterial / analysis
Disease Outbreaks
Infant, Newborn
Nurseries, Hospital
Staphylococcal Infections / epidemiology,  microbiology*
Staphylococcus aureus / classification*,  genetics
Reg. No./Substance:
0/DNA, Bacterial; EC 3.1.21.-/DNA Restriction Enzymes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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