Document Detail

The uterotonic activity of compounds isolated from the supercritical fluid extract of Ekebergia capensis.
MedLine Citation:
PMID:  11108547     Owner:  NLM     Status:  MEDLINE    
The wood of Ekebergia capensis Sparrm. is used by the local Zulu community in KwaZulu-Natal Province, South Africa to facilitate childbirth. In this investigation, the uterotonic properties of extracts from this tree were evaluated using both pregnant and non-pregnant guinea pig uterine smooth muscle in vitro. The extracts were prepared using water modified supercritical carbon dioxide at 400 atm and 80 degrees C. As samples of these extracts displayed positive results when screened for uterotonic activity, gravity column chromatography followed by NMR spectroscopy was performed in an attempt to isolate and elucidate the structures of the compounds that were present in the extract. The extract yielded five known compounds of which only two, viz. oleanonic acid and 3-epioleanolic acid, displayed uterotonic activity. Receptor binding assays were subsequently performed with 3-epioleanolic acid to ascertain its mode of action. Bradykinin (30 ng/100 microl) and acetylcholine (1 microg/100 microl) were used as the B2 and cholinergic receptor agonists respectively with icatibant (HOE 140) (30 ng/100 microl) and atropine (60 micro/100 microl) as their corresponding antagonists. 3-epioleanolic acid was observed to mediate its effect through the cholinergic receptor. The results of this study show that two compounds from the extract of this tree possess varying degrees of agonist activity on uterine smooth muscle with minor changes in the molecular structure affecting its intrinsic activity on uterine muscle.
V Sewram; M W Raynor; D A Mulholland; D M Raidoo
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of pharmaceutical and biomedical analysis     Volume:  24     ISSN:  0731-7085     ISO Abbreviation:  J Pharm Biomed Anal     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2001-03-08     Completed Date:  2001-05-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8309336     Medline TA:  J Pharm Biomed Anal     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  133-45     Citation Subset:  IM    
Department of Chemistry and Applied Chemistry, University of Natal, Durban, South Africa.
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MeSH Terms
Chromatography, High Pressure Liquid
Guinea Pigs
Plant Extracts / pharmacology
Plants, Medicinal / chemistry*
Pyrilamine / pharmacology
Receptor, Bradykinin B2
Receptors, Bradykinin / physiology
Receptors, Cholinergic / drug effects,  physiology
Uterine Contraction / drug effects*
Reg. No./Substance:
0/Plant Extracts; 0/Receptor, Bradykinin B2; 0/Receptors, Bradykinin; 0/Receptors, Cholinergic; 91-84-9/Pyrilamine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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