Document Detail


The usefulness of the lactate dehydrogenase macroreaction in autopsy practice.
MedLine Citation:
PMID:  8302818     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The detection of recent myocardial damage by means of the macroscopic lactate dehydrogenase reaction (LDHr) linked to nicotinamide adenine dinucleotide diaphorase (NADHd), conducted in a liquid medium is greatly insensitive to postmortal autolysis. In 10 heart cones (i.e., cardiac ventricles severed underneath the coronary sulcus) stored at 6 degrees C for up to 114 h after death, no autolytic artifacts appeared on freshly cut apical surfaces of transverse myocardial slices. In 10 cones kept at room temperature for up to 95 h after death, no artifacts appeared in eight cases; in two cases the LDHr was impaired by postmortal bacterial spread and decomposition of the myocardium. Intravital perfusion of injured myocardium increases the sensitivity of the LDHr. Postmortal stand-still of circulation is decisive in preserving dehydrogenase activities in undamaged myocardium. An artificial decrease in enzyme activity always appeared on the nonrecent, basally facing cut surfaces of slices exposed to air and fluid oozing out of the myocardium for long periods, even if the exposed surface of the cone was kept at 6 degrees C and wrapped in plastic. In normal practice, when bodies are stored in a refrigerating unit, the LDHr may still indicate myocardial damage more than 114 h after death.
Authors:
K Kubat; F Smedts
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc     Volume:  6     ISSN:  0893-3952     ISO Abbreviation:  Mod. Pathol.     Publication Date:  1993 Nov 
Date Detail:
Created Date:  1994-03-04     Completed Date:  1994-03-04     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8806605     Medline TA:  Mod Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  743-7     Citation Subset:  IM    
Affiliation:
Department of Pathology, University Hospital Nijmegen, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Aged, 80 and over
Artifacts
Autolysis
Autopsy / methods*
Dihydrolipoamide Dehydrogenase / metabolism
Evaluation Studies as Topic
Female
Histocytochemistry
Humans
L-Lactate Dehydrogenase / metabolism*
Male
Middle Aged
Myocardium / enzymology*,  pathology*
Postmortem Changes
Reproducibility of Results
Temperature
Time Factors
Tissue Preservation
Chemical
Reg. No./Substance:
EC 1.1.1.27/L-Lactate Dehydrogenase; EC 1.8.1.4/Dihydrolipoamide Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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