Document Detail


The usefulness of in vitro models to predict the bioavailability of iron and zinc: a consensus statement from the HarvestPlus expert consultation.
MedLine Citation:
PMID:  16711470     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A combination of dietary and host-related factors determines iron and zinc absorption, and several in vitro methods have been developed as preliminary screening tools for assessing bioavailability. An expert committee has reviewed evidence for their usefulness and reached a consensus. Dialyzability (with and without simulated digestion) gives some useful information but cannot predict the correct magnitude of response and may sometimes predict the wrong direction of response. Caco-2 cell systems (with and without simulated digestion) have been developed for iron availability, but the magnitude of different effects does not always agree with results obtained in human volunteers, and the data for zinc are too limited to draw conclusions about the validity of the method. Caco-2 methodologies vary significantly between laboratories and require experienced technicians and good quality cell culture facilities to obtain reproducible results. Algorithms can provide semi-quantitative information enabling diets to be classified as high, moderate, or low bioavailability. While in vitro methods can be used to generate ideas and develop hypotheses, they cannot be used alone for important decisions concerning food fortification policy, selection of varieties for plant breeding programs, or for new product development in the food industry. Ultimately human studies are required for such determinations.
Authors:
Sue Fairweather-Tait; Sean Lynch; Christine Hotz; Richard Hurrell; Leo Abrahamse; Steve Beebe; Stine Bering; Klaus Bukhave; Ray Glahn; Michael Hambidge; Janet Hunt; Bo Lonnerdal; Denis Miller; Najat Mohktar; Penelope Nestel; Manju Reddy; Ann-Sofie Sandber; Paul Sharp; Birgit Teucher; Trinidad P Trinidad
Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  International journal for vitamin and nutrition research. Internationale Zeitschrift für Vitamin- und Ernährungsforschung. Journal international de vitaminologie et de nutrition     Volume:  75     ISSN:  0300-9831     ISO Abbreviation:  Int J Vitam Nutr Res     Publication Date:  2005 Nov 
Date Detail:
Created Date:  2006-05-22     Completed Date:  2006-09-21     Revised Date:  2007-02-21    
Medline Journal Info:
Nlm Unique ID:  1273304     Medline TA:  Int J Vitam Nutr Res     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  371-4     Citation Subset:  IM    
Affiliation:
Institute of Food Research, Norwich Research Park, Norfolk, UK. sue.fairweather-tait@bbsrc.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Biological Availability
Caco-2 Cells
Humans
Iron / metabolism,  pharmacokinetics*
Zinc / metabolism,  pharmacokinetics*
Chemical
Reg. No./Substance:
7439-89-6/Iron; 7440-66-6/Zinc

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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