| The use of xanthine oxidase for a specific and sensitive fluorimetric determination of 6-mercaptopurine in serum. | |
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MedLine Citation:
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PMID: 6549126 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A specific and highly sensitive fluorimetric method was developed for the determination of 6-mercaptopurine (6MP) in serum. The method is based on the enzymatic oxidation of 6MP with xanthine oxidase to the oxypurine, followed by oxidation with acidic chromate to the corresponding 6-sulfonate. The fluorescent product has excitation and emission maxima at 330 and 400 nm, respectively. The limit of sensitivity was approximately 22 pg/ml for 6MP in water. The sensitivity limit for 6MP in serum containing azathioprine was approximately 2.2 ng/ml. The rate constants for conversion of 6MP into the final product (6-thiouric acid) and the apparent Michaelis constant were also determined by a nonlinear regression analysis based on the integrated Michaelis-Menten equation using the ultraviolet absorbance data and the simplified complementary tristimulus colorimetry. |
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Authors:
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R Tawa; S Takeshima; S Hirose |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochemical medicine Volume: 32 ISSN: 0006-2944 ISO Abbreviation: Biochem Med Publication Date: 1984 Dec |
Date Detail:
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Created Date: 1985-02-04 Completed Date: 1985-02-04 Revised Date: 2009-11-11 |
Medline Journal Info:
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Nlm Unique ID: 0151424 Medline TA: Biochem Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 303-10 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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6-Mercaptopurine
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blood* Chromatography, High Pressure Liquid Humans Kinetics Oxidation-Reduction Spectrometry, Fluorescence Uric Acid / analogs & derivatives, biosynthesis Xanthine Oxidase* |
| Chemical | |
Reg. No./Substance:
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2002-60-0/6-thiouric acid; 50-44-2/6-Mercaptopurine; 69-93-2/Uric Acid; EC 1.17.3.2/Xanthine Oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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