Document Detail


The use of xanthine oxidase for a specific and sensitive fluorimetric determination of 6-mercaptopurine in serum.
MedLine Citation:
PMID:  6549126     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A specific and highly sensitive fluorimetric method was developed for the determination of 6-mercaptopurine (6MP) in serum. The method is based on the enzymatic oxidation of 6MP with xanthine oxidase to the oxypurine, followed by oxidation with acidic chromate to the corresponding 6-sulfonate. The fluorescent product has excitation and emission maxima at 330 and 400 nm, respectively. The limit of sensitivity was approximately 22 pg/ml for 6MP in water. The sensitivity limit for 6MP in serum containing azathioprine was approximately 2.2 ng/ml. The rate constants for conversion of 6MP into the final product (6-thiouric acid) and the apparent Michaelis constant were also determined by a nonlinear regression analysis based on the integrated Michaelis-Menten equation using the ultraviolet absorbance data and the simplified complementary tristimulus colorimetry.
Authors:
R Tawa; S Takeshima; S Hirose
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemical medicine     Volume:  32     ISSN:  0006-2944     ISO Abbreviation:  Biochem Med     Publication Date:  1984 Dec 
Date Detail:
Created Date:  1985-02-04     Completed Date:  1985-02-04     Revised Date:  2009-11-11    
Medline Journal Info:
Nlm Unique ID:  0151424     Medline TA:  Biochem Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  303-10     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
6-Mercaptopurine / blood*
Chromatography, High Pressure Liquid
Humans
Kinetics
Oxidation-Reduction
Spectrometry, Fluorescence
Uric Acid / analogs & derivatives,  biosynthesis
Xanthine Oxidase*
Chemical
Reg. No./Substance:
2002-60-0/6-thiouric acid; 50-44-2/6-Mercaptopurine; 69-93-2/Uric Acid; EC 1.17.3.2/Xanthine Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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