Document Detail


The use of wideband filters in distinguish green fluorescent protein in roots of arbuscular mycorrhizal symbiosis.
MedLine Citation:
PMID:  20818177     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Arbuscular mycorrhizal (AM) fungi form arbuscules in the inner cortical cells of roots. Accumulation of autofluorescent materials within the roots, especially around senescent arbuscules, has hampered analyses of the localization and dynamics of green fluorescent protein (GFP)-fusion proteins in arbusculated cells. In this report, the author proposes an efficient method to distinguish GFP from autofluorescence. To detect GFP fluorescence, colonized cells were observed with wideband filters rather than GFP-specialized filters because the autofluorescence generally has a broad fluorescent spectrum that can easily be distinguished from GFP by color. Moreover, the autofluorescence of arbusculated cells could possibly contain strong green fluorescence that could not be excluded by GFP-specialized filters. The multicolor imaging and in vivo real-time observations suggested that the expression of autofluorescence in arbusculated cells did not overlap with the expression of OsPT11-GFP, a useful marker for active arbuscules, and that autofluorescent materials appeared after the initiation of senescence in infection units.
Authors:
Yoshihiro Kobae
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-01
Journal Detail:
Title:  Plant signaling & behavior     Volume:  5     ISSN:  1559-2324     ISO Abbreviation:  Plant Signal Behav     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2011-04-14     Completed Date:  2011-12-07     Revised Date:  2013-05-28    
Medline Journal Info:
Nlm Unique ID:  101291431     Medline TA:  Plant Signal Behav     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1150-2     Citation Subset:  IM    
Affiliation:
Laboratory of Crop Science, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan. kobae@agr.nagoya-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Cell Aging / physiology
Color
Fluorescence*
Green Fluorescent Proteins / physiology*
Microscopy, Fluorescence / methods*
Mycorrhizae / physiology*
Oryza sativa / metabolism*
Plant Roots / physiology*
Symbiosis / physiology*
Chemical
Reg. No./Substance:
147336-22-9/Green Fluorescent Proteins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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