Document Detail

The use of a reference allergenic extract in the evaluation of allergen products.
MedLine Citation:
PMID:  4003725     Owner:  NLM     Status:  MEDLINE    
Two commercially available mugwort (Artemisia vulgaris (Av] pollen extracts Av-ALK and Av-PS were compared with a dialysed reference mugwort pollen extract (Av-REF) by means of crossed immunoelectrophoresis (CIE), crossed radio immunoelectrophoresis (CRIE), RAST-inhibition, RAST-screening and skin prick test. Av-ALK and Av-PS both contained approximately half the number of precipitation arcs detectable in Av-REF, and Av-PS seemed to lack two intermediate allergens and two minor allergens, while Av-ALK seemed to lack one minor allergen. The RAST-inhibition curve obtained for Av-PS did not parallel the curves obtained for Av-ALK and Av-REF, indicating that the allergen composition of Av-PS differs from that of the others. Further, RAST-screening experiments support the CIE observation that Av-PS seemed to lack two of the intermediate allergens. The HEP values determined for Av-ALK and Av-PS diverge considerably from those stated by the manufacturers. The results obtained demonstrate that the outcome of a comparison between a reference allergenic extract and allergenic products depends very much on the method employed.
L Ingemann; H Formgren; H Løwenstein; H Ipsen
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Allergy     Volume:  40     ISSN:  0105-4538     ISO Abbreviation:  Allergy     Publication Date:  1985 May 
Date Detail:
Created Date:  1985-07-24     Completed Date:  1985-07-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7804028     Medline TA:  Allergy     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  273-81     Citation Subset:  IM    
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MeSH Terms
Allergens / analysis,  standards*
Immunoelectrophoresis, Two-Dimensional
Pollen / immunology
Radioallergosorbent Test
Reference Standards
Skin Tests
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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