Document Detail

The use of rats associated with a human faecal flora as a model for studying the effects of diet on the human gut microflora.
MedLine Citation:
PMID:  2820914     Owner:  NLM     Status:  MEDLINE    
The activities of four bacterial biotransformation enzymes (beta-glucosidase, beta-glucuronidase, nitrate reductase and nitroreductase) were measured in the caecal contents of conventional flora rats or germ-free rats contaminated with a mixed, human faecal flora and compared with activities present in a fresh human stool preparation. Both the conventional flora rats and the rats inoculated with a human flora exhibited an enzyme profile generally similar to that of human faeces, although the conventional rat flora exhibited negligible nitrate reductase activity. The enzyme profile remained essentially unaltered in both human flora preparations following supplementation of the diet with pectin, whereas the conventional rat flora responded to this plant cell wall carbohydrate with a significant increase in nitrate reductase activity. The results demonstrate that enzymic activities of the human faecal microflora can be simulated in rats associated with a mixed population of human intestinal bacteria.
A K Mallett; C A Bearne; I R Rowland; M J Farthing; C B Cole; R Fuller
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of applied bacteriology     Volume:  63     ISSN:  0021-8847     ISO Abbreviation:  J. Appl. Bacteriol.     Publication Date:  1987 Jul 
Date Detail:
Created Date:  1987-11-19     Completed Date:  1987-11-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7503050     Medline TA:  J Appl Bacteriol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  39-45     Citation Subset:  IM    
British Industrial Biological Research Association, Carshalton, Surrey, UK.
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MeSH Terms
Analysis of Variance
Bacteria / enzymology*,  growth & development
Cecum / microbiology
Dietary Fiber / metabolism
Feces / microbiology*
Germ-Free Life
Glucuronidase / metabolism
Models, Biological*
Nitrate Reductases / metabolism
Nitroreductases / metabolism
Pectins / metabolism
beta-Glucosidase / metabolism
Reg. No./Substance:
0/Pectins; 9000-69-5/pectin; EC 1.7.-/Nitrate Reductases; EC 1.7.-/Nitroreductases; EC; EC

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