Document Detail

The use of radiolabelling techniques to measure substantivity to, and penetration into, hair of protein hydrolysates.
MedLine Citation:
PMID:  18507628     Owner:  NLM     Status:  In-Data-Review    
The use of 14C-labelled amino acids enables the measurement of both the total substantivity to hair and the degree of penetration into the hair shaft of amino acid mixtures derived from complete hydrolysis of proteins. The technique utilizes the fact that direct measurement of 14C radioactivity of the treated hair detects only the surface substantivity. Total substantivity can be determined following solubilization of the hair. Data obtained for wheat amino acids show significant penetration when used to treat hair from a shampoo or conditioner formulation. A similar technique has been investigated for a wheat protein partial hydrolysate using 14CNO for radiolabelling purposes and shows that significant penetration into hair can occur. L'utilisation d'amino-acides marques au 14C permet la mesure a la fois de l'absorption totale par les cheveux et du degre de penetration dans la fibre du cheveu de melanges d'amino-acides obtenus a partir d'une hydrolyse totale de proteines. La technique utilise le fait que la mesure directe de la radioactivite du 14C des cheveux traites ne detecte que l'absorption en surface. L'absorption totale peut etre determinee apres solubilisation des cheveux. Les donnees obtenues a partir d'amino-acides de ble montrent une penetration significative lors d'une utilisation pour traiter des cheveux a partir d'une formulation de shampoing ou d'apres-shampoing. Une technique similaire a ete exploree vis-a-vis d'un hydrolysat partiel d'une proteine de ble utilisant 14CNO a des fins de marquage, et montre qu'une penetration significative dans les cheveux peut avoir lieu.
R T Jones; S P Chahal
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of cosmetic science     Volume:  19     ISSN:  0142-5463     ISO Abbreviation:  Int J Cosmet Sci     Publication Date:  1997 Oct 
Date Detail:
Created Date:  2008-05-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8007161     Medline TA:  Int J Cosmet Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  215-26     Citation Subset:  -    
Croda Colloids Ltd, Foundry Lane, Ditton, Widnes Cheshire UK.
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