Document Detail

The use of post-embedding immunoelectron microscopy in the diagnosis of glomerular diseases. Comparison of immunoelectron microscopic and immunofluorescence studies.
MedLine Citation:
PMID:  1472359     Owner:  NLM     Status:  MEDLINE    
Fifty renal biopsies were studied by immunoelectron microscopy after embedding in a partly hydrophilic polyacrylic resin (LR White). Immunofluorescence studies were carried out on frozen sections of parallel tissue samples. Polyacrylic embedding gave good preservation of the renal ultrastructure and precise localization of immunoglobulin and C3c antibodies within glomerular electron-dense deposits. Non-specific staining of plasma proteins within vascular lumina could easily be detected. There was good correlation between immunoelectron and immunofluorescence microscopy. Immunoelectron microscopy is a very sensitive method, which can detect small amounts of antigen. More cases were, however, positive by immunofluorescence than by immunoelectron microscopy. This discrepancy may be explained by difference in sample size, and by difference in resolution of morphological details (electron microscopy versus fluorescence microscopy).
F J Skjørten; S H Brorson; B Roald; E H Strøm; B Lund
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  APMIS : acta pathologica, microbiologica, et immunologica Scandinavica     Volume:  100     ISSN:  0903-4641     ISO Abbreviation:  APMIS     Publication Date:  1992 Nov 
Date Detail:
Created Date:  1993-02-04     Completed Date:  1993-02-04     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8803400     Medline TA:  APMIS     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  1001-7     Citation Subset:  IM    
Department of Pathology, Ullevaal Hospital, Oslo, Norway.
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MeSH Terms
Acrylic Resins
Fluorescent Antibody Technique
Glomerulonephritis / diagnosis
Hypertension, Renal / diagnosis
Immunoglobulins / metabolism
Kidney Diseases / diagnosis*
Kidney Glomerulus / immunology,  ultrastructure
Microscopy, Immunoelectron / methods*
Tissue Embedding
Reg. No./Substance:
0/Acrylic Resins; 0/Immunoglobulins

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