| The use of post-embedding immunoelectron microscopy in the diagnosis of glomerular diseases. Comparison of immunoelectron microscopic and immunofluorescence studies. | |
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MedLine Citation:
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PMID: 1472359 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fifty renal biopsies were studied by immunoelectron microscopy after embedding in a partly hydrophilic polyacrylic resin (LR White). Immunofluorescence studies were carried out on frozen sections of parallel tissue samples. Polyacrylic embedding gave good preservation of the renal ultrastructure and precise localization of immunoglobulin and C3c antibodies within glomerular electron-dense deposits. Non-specific staining of plasma proteins within vascular lumina could easily be detected. There was good correlation between immunoelectron and immunofluorescence microscopy. Immunoelectron microscopy is a very sensitive method, which can detect small amounts of antigen. More cases were, however, positive by immunofluorescence than by immunoelectron microscopy. This discrepancy may be explained by difference in sample size, and by difference in resolution of morphological details (electron microscopy versus fluorescence microscopy). |
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Authors:
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F J Skjørten; S H Brorson; B Roald; E H Strøm; B Lund |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica Volume: 100 ISSN: 0903-4641 ISO Abbreviation: APMIS Publication Date: 1992 Nov |
Date Detail:
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Created Date: 1993-02-04 Completed Date: 1993-02-04 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 8803400 Medline TA: APMIS Country: DENMARK |
Other Details:
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Languages: eng Pagination: 1001-7 Citation Subset: IM |
Affiliation:
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Department of Pathology, Ullevaal Hospital, Oslo, Norway. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acrylic Resins Biopsy Fluorescent Antibody Technique Glomerulonephritis / diagnosis Humans Hypertension, Renal / diagnosis Immunoglobulins / metabolism Kidney Diseases / diagnosis* Kidney Glomerulus / immunology, ultrastructure Microscopy, Immunoelectron / methods* Tissue Embedding |
| Chemical | |
Reg. No./Substance:
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0/Acrylic Resins; 0/Immunoglobulins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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