Document Detail

The use of poly(2-acrylamido-2-methyl-1-propanesulfonic acid) polymers as spacers for isotachophoresis in sieving gel matrices.
MedLine Citation:
PMID:  10036157     Owner:  NLM     Status:  MEDLINE    
The electric field strength gradients generated in isotachophoresis (ITP) may be used for the separation of biomolecules. Poly(2-acrylamido-2-methyl-1-propanesulfonic acid) (polyAMPS) polymers of a uniform distribution of molecular mass were synthesized and used as novel spacers in ITP. Since these polymeric spacers are strongly acidic species, their ionic charges remain constant over a wide pH range, so that their ionic mobilities are governed solely by their molecular masses and not by the pH of the milieu. A modification of ITP known as telescope electrophoresis was used to separate a number of acidic dyes of varying ionic mobility, using polyAMPS polymers as spacers. The resolution obtained was superior to that obtained by polyacrylamide gel electrophoresis (PAGE), due to the focusing effect of the electric field strength gradient. Since these novel polymeric spacers are designed to operate within sieving medium, it was decided to test their suitability for the separation of DNA molecules. DNA molecules up to 1000 bp long were successfully resolved, with a similar resolution to that obtained with conventional PAGE.
M P Bellini; K L Manchester
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical biochemistry     Volume:  268     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1999 Mar 
Date Detail:
Created Date:  1999-04-27     Completed Date:  1999-04-27     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  21-9     Citation Subset:  IM    
Copyright Information:
Copyright 1999 Academic Press.
Department of Biochemistry, University of the Witwatersrand, Johannesburg, South Africa.
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MeSH Terms
Coloring Agents / chemistry
DNA / analysis
Electrophoresis, Polyacrylamide Gel / methods*
Polymers / chemistry*
Sulfonic Acids / chemistry*
Thioglycolates / chemistry
Reg. No./Substance:
0/Coloring Agents; 0/Polymers; 0/Sulfonic Acids; 0/Thioglycolates; 27119-07-9/poly(2-acrylamido-2-methyl-1-propanesulfonic acid); 9007-49-2/DNA

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