Document Detail


The use of plasmatic acceptors as specific ligands in affinity chromatography cleanup of veterinary drugs from biological matrices.
MedLine Citation:
PMID:  18193407     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bovine alpha(1)-acid glycoprotein (bAAG) and bovine serum albumin (BSA) are plasmatic acceptors working as carriers by the specific and reversible binding of several drugs in vivo. We synthesized affinity columns by coupling bAAG and BSA to an activated chromatographic support through their carbohydrate moieties, to preserve protein tertiary structure and, consequently, to improve the biological activity in vitro. The bAAG and BSA affinity columns were used to study the binding of acidic and basic drugs. Moreover, a purification strategy was developed for the cleanup of drug residues from biological matrices and foods, prior to screening and/or confirmatory analysis, on the basis of the specific molecular recognition between the protein and the drug. The aim of this work was to test the potency of bAAG- and BSA-based affinity chromatography to bind some veterinary drugs and purify them in the context of the official control of animal products. The efficiency of these homemade affinity columns in minimising matrix interference and in selective cleanup of different classes of substances was reported and discussed.
Authors:
Pasquale Gallo; Antonella Nasi; Floriana Vinci; Maurizio Fiori; Gianfranco Brambilla; Luigi Serpe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-01-11
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  391     ISSN:  1618-2650     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-23     Completed Date:  2008-06-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1153-62     Citation Subset:  IM    
Affiliation:
Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute 2, 80055, Portici (NA), Italy. pasquale.gallo@cert.izsmportici.it
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Chromatography, Affinity / instrumentation,  methods*
Chromatography, High Pressure Liquid
Drug Residues / isolation & purification
Ligands
Liver / metabolism
Mass Spectrometry
Orosomucoid / chemistry,  metabolism*
Reference Standards
Sensitivity and Specificity
Serum Albumin, Bovine / chemistry,  metabolism*
Solvents
Veterinary Drugs / isolation & purification*,  metabolism*
Chemical
Reg. No./Substance:
0/Ligands; 0/Orosomucoid; 0/Serum Albumin, Bovine; 0/Solvents; 0/Veterinary Drugs

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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