Document Detail


The use of phosphate buffered saline for the recovery of cells and spermatozoa from swabs.
MedLine Citation:
PMID:  17388245     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the forensic science laboratory, the recovery of spermatozoa from vaginal swabs, or vaginal cells from penile swabs, can help determine if sexual intercourse may have taken place. There are several methods used to recover spermatozoa and cells from the swabs before visualisation on a microscope slide and most of these methods use water. Phosphate buffered saline (PBS) is a non-toxic solution used in many biological laboratories. Unlike water, PBS prevents cells rupturing or shrivelling up due to osmosis. This study demonstrates that PBS can be used for the extraction of spermatozoa and cells from swabs and that PBS does not affect subsequent DNA profiling.
Authors:
N C Martin; A A Pirie; L V Ford; C L Callaghan; K McTurk; D Lucy; D G Scrimger
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Science & justice : journal of the Forensic Science Society     Volume:  46     ISSN:  1355-0306     ISO Abbreviation:  Sci. Justice     Publication Date:    2006 Jul-Sep
Date Detail:
Created Date:  2007-03-28     Completed Date:  2007-10-12     Revised Date:  2007-10-19    
Medline Journal Info:
Nlm Unique ID:  9508563     Medline TA:  Sci Justice     Country:  England    
Other Details:
Languages:  eng     Pagination:  179-84     Citation Subset:  IM    
Affiliation:
Forensic Science Support Department, Lothian & Borders Police, 11 Howdenhall Road, Edinburgh.
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MeSH Terms
Descriptor/Qualifier:
Buffers
DNA / analysis
Female
Humans
Male
Phosphates / chemistry*
Potassium Compounds / chemistry
Semen
Sodium Chloride / chemistry*
Specimen Handling / methods
Spermatozoa*
Vagina / cytology
Chemical
Reg. No./Substance:
0/Buffers; 0/Phosphates; 0/Potassium Compounds; 16068-46-5/potassium phosphate; 7632-05-5/sodium phosphate; 7647-14-5/Sodium Chloride; 9007-49-2/DNA
Comments/Corrections
Erratum In:
Sci Justice. 2007 Sep;47(2):108

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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