| The use of phosphate buffered saline for the recovery of cells and spermatozoa from swabs. | |
MedLine Citation:
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PMID: 17388245 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the forensic science laboratory, the recovery of spermatozoa from vaginal swabs, or vaginal cells from penile swabs, can help determine if sexual intercourse may have taken place. There are several methods used to recover spermatozoa and cells from the swabs before visualisation on a microscope slide and most of these methods use water. Phosphate buffered saline (PBS) is a non-toxic solution used in many biological laboratories. Unlike water, PBS prevents cells rupturing or shrivelling up due to osmosis. This study demonstrates that PBS can be used for the extraction of spermatozoa and cells from swabs and that PBS does not affect subsequent DNA profiling. |
Authors:
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N C Martin; A A Pirie; L V Ford; C L Callaghan; K McTurk; D Lucy; D G Scrimger |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Science & justice : journal of the Forensic Science Society Volume: 46 ISSN: 1355-0306 ISO Abbreviation: Sci. Justice Publication Date: 2006 Jul-Sep |
Date Detail:
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Created Date: 2007-03-28 Completed Date: 2007-10-12 Revised Date: 2007-10-19 |
Medline Journal Info:
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Nlm Unique ID: 9508563 Medline TA: Sci Justice Country: England |
Other Details:
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Languages: eng Pagination: 179-84 Citation Subset: IM |
Affiliation:
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Forensic Science Support Department, Lothian & Borders Police, 11 Howdenhall Road, Edinburgh. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Buffers DNA / analysis Female Humans Male Phosphates / chemistry* Potassium Compounds / chemistry Semen Sodium Chloride / chemistry* Specimen Handling / methods Spermatozoa* Vagina / cytology |
| Chemical | |
Reg. No./Substance:
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0/Buffers; 0/Phosphates; 0/Potassium Compounds; 16068-46-5/potassium phosphate; 7632-05-5/sodium phosphate; 7647-14-5/Sodium Chloride; 9007-49-2/DNA |
| Comments/Corrections | |
Erratum In:
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Sci Justice. 2007 Sep;47(2):108 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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