Document Detail

The use of periovulatory human cervical mucus in human sperm capacitation and in-vitro fertilization of foreign eggs.
MedLine Citation:
PMID:  6670968     Owner:  NLM     Status:  MEDLINE    
The time requirement of a routine in-vitro fertilization experiment using human spermatozoa and zona-free hamster oocytes is more than one working day. We have used a simple process of simultaneous sperm washing and capacitation before challenging them against oocytes. Spermatozoa were recovered up to 90 minutes from the proximal end of a column of periovulatory human cervical mucus into Brinster, Whitten and Whittingham's (B.W.W.) medium. Without any further capacitation 50 microliters of these spermatozoal suspension, in the concentration of 2.5-3 X 10(6)/ml, fertilized 81% of a total of 100 eggs. A dramatic decrease in the fertilization rate (18%) was observed when these spermatozoa were further capacitated in-vitro for five hours before actual egg incubation for three hours. Both selection and accelerated capacitation may contribute to this result.
A K Siddiquey; J R Newton
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Bangladesh Medical Research Council bulletin     Volume:  9     ISSN:  0377-9238     ISO Abbreviation:  Bangladesh Med Res Counc Bull     Publication Date:  1983 Jun 
Date Detail:
Created Date:  1984-04-11     Completed Date:  1984-04-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7607686     Medline TA:  Bangladesh Med Res Counc Bull     Country:  BANGLADESH    
Other Details:
Languages:  eng     Pagination:  25-31     Citation Subset:  IM    
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MeSH Terms
Cervix Mucus / physiology*
Fertilization in Vitro / methods*
Oocytes / growth & development
Ovum / physiology
Sperm Capacitation*
Spermatozoa / growth & development

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