| The use of paraffin-processed tissue and the immunoperoxidase technique in the diagnosis of bullous diseases, lupus erythematosus and vasculitis. | |
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MedLine Citation:
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PMID: 7041947 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Twenty-two skin biopsies have been split and each half examined either by the immunoperoxidase technique on paraffin-fixed tissue or by both immunoperoxidase (IP) and immunofluorescence (IF) technique on snap-frozen tissue. All twenty-two specimens gave positive results by frozen IF techniques, nineteen out of twenty-two (86%) were positive by frozen IP techniques and sixteen out of twenty-two (72%) were positive by IP on paraffin-fixed material. If retrospective immunopathology is required on a specimen and only paraffin-fixed material is available a positive result may be expected in the majority of cases. |
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Authors:
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M L Turbitt; R M Mackie; H Young; I Campbell |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The British journal of dermatology Volume: 106 ISSN: 0007-0963 ISO Abbreviation: Br. J. Dermatol. Publication Date: 1982 Apr |
Date Detail:
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Created Date: 1982-07-19 Completed Date: 1982-07-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0004041 Medline TA: Br J Dermatol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 411-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Dermatitis Herpetiformis
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diagnosis Fluorescent Antibody Technique Frozen Sections Humans Immunoenzyme Techniques Lupus Erythematosus, Discoid / diagnosis* Lupus Erythematosus, Systemic / diagnosis* Paraffin Pemphigoid, Bullous / diagnosis Pemphigus / diagnosis Skin Diseases / diagnosis* Skin Diseases, Vesiculobullous / diagnosis* Vasculitis / diagnosis* |
| Chemical | |
Reg. No./Substance:
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8002-74-2/Paraffin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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