Document Detail


The use of non-physiological conditions to isolate fetal cells from maternal blood.
MedLine Citation:
PMID:  15561097     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fetal cells are always present in maternal blood starting in the first trimester of pregnancy, however a rapid, simple, and consistent procedure for their isolation for prenatal non-invasive genetic investigation is still lacking. Sensitivity and recovery of fetal cells is jeopardized by the minute amount of circulating fetal cells and their loss during the enrichment procedure. We report here a single-step approach to isolate fetal cells from maternal blood which relies on the use of non-physiological conditions to modify cell densities before their separation in a density gradient and in a newly developed cell separation device. Isolated fetal cells have been investigated using cytochemistry, Soret band absorption microscopy, monoclonal antibodies for epsilon- and gamma-chain-Hb, monoclonal antibody for i-antigen, and by fluorescence in situ hybridization (FISH). Fetal cells were always detected in all 105 maternal blood samples investigated and fetal aneuploidies were correctly diagnosed by FISH, in a pilot study of pathological pregnancies, in fetal cells isolated from maternal blood obtained either before or after invasive procedure.
Authors:
Giammaria Sitar; Bruno Brambati; Marina Baldi; Laura Montanari; Massimo Vincitorio; Lucia Tului; Antonino Forabosco; Edoardo Ascari
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental cell research     Volume:  302     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2004-11-24     Completed Date:  2005-01-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  153-61     Citation Subset:  IM    
Affiliation:
Department of Medicine Policlinico San Matteo and University of Pavia, Italy. g.sitar@smatteo.pv.it
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MeSH Terms
Descriptor/Qualifier:
Aneuploidy
Antibodies, Monoclonal / metabolism
Blood Cells / cytology*
Cell Separation / methods*
Female
Fetal Blood / cytology*
Fetal Diseases / pathology
Fetal Hemoglobin / metabolism
Hematopoietic Stem Cells / cytology*
Humans
Immunohistochemistry
In Situ Hybridization, Fluorescence
Male
Pilot Projects
Polymerase Chain Reaction
Pregnancy
Pregnancy Trimester, First
Prenatal Diagnosis / methods*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 9034-63-3/Fetal Hemoglobin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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