Document Detail


The use of multidrug approach to uncover new players of the endomembrane system trafficking machinery.
MedLine Citation:
PMID:  24306870     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Chemical Biology is a strong tool to perform experimental procedures to study the Endomembrane System (ES) in plant biology. In the last few years, several bioactive compounds and their effects upon protein trafficking as well as organelle distribution, identity, and size in plants and yeast have been characterized. Today, several of these chemical tools are widely used to perform mutant screens and establish the trafficking pathway of a given cellular component. This chapter is a guideline to perform multidrug approaches to study the endomembrane system in plant cells. This type of approach is a powerful and useful strategy to thoroughly determine the trafficking of a specific protein as well as to perform mutant screens based on phenotypes produced by drug treatments. On the other hand, a multidrug approach can address the characterization of a new bioactive molecule and find its cellular pathway target. Overall, this approach can unravel mechanisms and identify new players in endomembrane trafficking.
Authors:
Daniela Urbina; Patricio Pérez-Henríquez; Lorena Norambuena
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  1056     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2014  
Date Detail:
Created Date:  2013-12-05     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  131-43     Citation Subset:  IM    
Affiliation:
Plant Molecular Biology Laboratory, Department of Biology, Faculty of Sciences, University of Chile, Santiago, Chile.
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