Document Detail


The use of monoreassortants and reverse genetics to map reovirus lysis of a ras-transformed cell line.
MedLine Citation:
PMID:  17049626     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Reovirus has been shown to lyse most transformed cells while establishing a persistent or abortive infection in non-transformed cells. Developing methods to identify the reovirus genes associated with oncolysis is an important step toward understanding the mechanisms involved. This report is the first to develop and apply the use of monoreassortants and reverse genetics to identify an individual reovirus gene associated with reovirus oncolysis. Infection with reovirus serotypes 1/Lang, 2/Jones or 3/Dearing of cells transformed with a normal copy of c-Ha-RAS (N1 cells) or with a normal copy of c-Myc (Myc-3 cells), produces large amounts of progeny virus of all three serotypes and results in lysis of both these cell lines. Infection of cells transformed with a mutant c-Ha-RAS gene (T1 cells) with either serotype 1/Lang and 2/Jones results in the production of large amounts of virus and lysis of the cells. In sharp contrast, serotype 3/Dearing virus infection of these cells produced small amounts of virus and resulted in limited lysis of these cells. Using monoreassortants and reverse genetics we exploited this phenotypic difference between the three serotypes to identify a single reovirus gene linked to the preferential lysis of the T1 cells, the S4 gene.
Authors:
Michael R Roner; Christine Mutsoli
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-10-17
Journal Detail:
Title:  Journal of virological methods     Volume:  139     ISSN:  0166-0934     ISO Abbreviation:  J. Virol. Methods     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-01-16     Completed Date:  2007-05-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8005839     Medline TA:  J Virol Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  132-42     Citation Subset:  IM    
Affiliation:
Department of Biology, The University of Texas Arlington, Arlington, TX 76019, USA. roner@uta.edu
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MeSH Terms
Descriptor/Qualifier:
Cell Line, Transformed / virology
Genetic Techniques*
Reassortant Viruses / genetics*
Reoviridae / genetics,  metabolism*,  physiology
Tumor Cells, Cultured
ras Proteins / genetics*,  physiology
Chemical
Reg. No./Substance:
EC 3.6.5.2/ras Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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