Document Detail


The use of indium-111 for studies of cytotoxicity mediated by lymphocytes or by antibodies and complement.
MedLine Citation:
PMID:  501729     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mouse P815 mastocytoma cells, Meth A sarcoma cells and human MRC5 fibroblasts and peripheral blood mononuclear cells were labelled with 111In, 51Cr or 125IUdR and their release from the cells measured. Spontaneous release of 111In from the cells in cultures was less than half that of 51Cr and was comparable to the release of 125I. When 111In-labelled P815 cells were lysed with antibodies and complement or with sensitized lymphocytes the label was released rapidly. 111In is a convenient label in short- as well as in long-term in vitro cytotoxicity studies because of its high labelling and detecting efficiency and its stable cell association.
Authors:
J Ferluga; A C Allison; M L Thakur
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of clinical & laboratory immunology     Volume:  1     ISSN:  0141-2760     ISO Abbreviation:  J Clin Lab Immunol     Publication Date:  1979 Feb 
Date Detail:
Created Date:  1980-01-19     Completed Date:  1980-01-19     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  7808987     Medline TA:  J Clin Lab Immunol     Country:  ITALY    
Other Details:
Languages:  eng     Pagination:  339-42     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies*
Cells, Cultured
Chromium Radioisotopes
Complement System Proteins*
Cytotoxicity, Immunologic*
Guinea Pigs
Humans
Indium / diagnostic use*
Iodine Radioisotopes
Lymphocytes / immunology*
Mice
Neoplasms, Experimental / immunology
Rabbits
Radioisotopes / diagnostic use*
Chemical
Reg. No./Substance:
0/Antibodies; 0/Chromium Radioisotopes; 0/Iodine Radioisotopes; 0/Radioisotopes; 7440-74-6/Indium; 9007-36-7/Complement System Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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