| The use of gel electrophoresis to study the reactions of activated amino acids with oligonucleotides. | |
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MedLine Citation:
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PMID: 11539447 Owner: NASA Status: MEDLINE |
Abstract/OtherAbstract:
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We have used gel electrophoresis to study the primary covalent addition of amino acids to oligonucleotides or their analogs and the subsequent addition of further molecules of the amino acids to generate peptides covalently linked to the oligonucleotides. We have surveyed the reactions of a variety of amino acids with the phosphoramidates derived from oligonucleotide 5'-phosphates and ethylenediamine. We find that arginine and amino acids that can interact with oligonucleotides through stacking interactions react most efficiently. D- and L-amino acids give indistinguishable families of products. |
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Authors:
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G Zieboll; L E Orgel |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Journal of molecular evolution Volume: 38 ISSN: 0022-2844 ISO Abbreviation: J. Mol. Evol. Publication Date: 1994 |
Date Detail:
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Created Date: 1997-08-02 Completed Date: 1997-08-02 Revised Date: 2007-07-17 |
Medline Journal Info:
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Nlm Unique ID: 0360051 Medline TA: J Mol Evol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 561-5 Citation Subset: S |
Affiliation:
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The Salk Institute for Biological Studies, San Diego, CA 92186-5800, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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chemistry* Electrophoresis, Polyacrylamide Gel / methods* Ethylenediamines / chemistry Evolution, Molecular Hydrogen-Ion Concentration Imidazoles / chemistry Oligonucleotides / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Ethylenediamines; 0/Imidazoles; 0/Oligonucleotides; 107-15-3/ethylenediamine; 530-62-1/N,N-carbonyldiimidazole |
| Investigator | |
Investigator/Affiliation:
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L E Orgel / U CA San Diego, La Jolla; S L Miller / U CA San Diego, La Jolla |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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