Document Detail


The use of gel electrophoresis to study the reactions of activated amino acids with oligonucleotides.
MedLine Citation:
PMID:  11539447     Owner:  NASA     Status:  MEDLINE    
Abstract/OtherAbstract:
We have used gel electrophoresis to study the primary covalent addition of amino acids to oligonucleotides or their analogs and the subsequent addition of further molecules of the amino acids to generate peptides covalently linked to the oligonucleotides. We have surveyed the reactions of a variety of amino acids with the phosphoramidates derived from oligonucleotide 5'-phosphates and ethylenediamine. We find that arginine and amino acids that can interact with oligonucleotides through stacking interactions react most efficiently. D- and L-amino acids give indistinguishable families of products.
Authors:
G Zieboll; L E Orgel
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of molecular evolution     Volume:  38     ISSN:  0022-2844     ISO Abbreviation:  J. Mol. Evol.     Publication Date:  1994  
Date Detail:
Created Date:  1997-08-02     Completed Date:  1997-08-02     Revised Date:  2007-07-17    
Medline Journal Info:
Nlm Unique ID:  0360051     Medline TA:  J Mol Evol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  561-5     Citation Subset:  S    
Affiliation:
The Salk Institute for Biological Studies, San Diego, CA 92186-5800, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / chemistry*
Electrophoresis, Polyacrylamide Gel / methods*
Ethylenediamines / chemistry
Evolution, Molecular
Hydrogen-Ion Concentration
Imidazoles / chemistry
Oligonucleotides / chemistry*
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Ethylenediamines; 0/Imidazoles; 0/Oligonucleotides; 107-15-3/ethylenediamine; 530-62-1/N,N-carbonyldiimidazole
Investigator
Investigator/Affiliation:
L E Orgel / U CA San Diego, La Jolla; S L Miller / U CA San Diego, La Jolla

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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