Document Detail

On the use of fluorescence probes for detecting reactive oxygen and nitrogen species associated with photodynamic therapy.
MedLine Citation:
PMID:  21054079     Owner:  NLM     Status:  MEDLINE    
Fluorescent probes are frequently employed for the detection of different reactive oxygen and nitrogen species formed during the irradiation of photosensitized cells and tissues. Investigators often interpret the results in terms of information provided with the different probes without examining specificity or determinants of fluorogenic reactions. We examine five fluorescent probes in a cell-free system: reduced 2',7'-dichlorofluorescein, dihydroethidine, dihydrorhodamine, 3'-(p aminophenyl) fluorescein (APF), and 4',5'-diaminofluorescein. Of these, only APF demonstrates a high degree of specificity for a single reactive species. There is a substantial influence of peroxidase activity on all fluorogenic interactions. The fluorescence of the photosensitizing agent also must be taken into account in evaluating results.
Michael Price; David Kessel
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  15     ISSN:  1560-2281     ISO Abbreviation:  J Biomed Opt     Publication Date:    2010 Sep-Oct
Date Detail:
Created Date:  2010-11-08     Completed Date:  2011-03-14     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  051605     Citation Subset:  IM    
Wayne State University School of Medicine, Cancer Biology Program, Detroit, Michigan, 48201, USA.
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MeSH Terms
Aniline Compounds
Cell Line, Tumor
Cell-Free System
Dicarbethoxydihydrocollidine / analogs & derivatives
Fluorescent Dyes*
Leukemia P388 / drug therapy,  metabolism
Microscopy, Fluorescence
Microscopy, Phase-Contrast
Reactive Nitrogen Species / metabolism*
Reactive Oxygen Species / metabolism*
Grant Support
CA 23378/CA/NCI NIH HHS; GM058905-11/GM/NIGMS NIH HHS; R01 CA023378/CA/NCI NIH HHS; R01 CA023378-30/CA/NCI NIH HHS
Reg. No./Substance:
0/Aniline Compounds; 0/Fluoresceins; 0/Fluorescent Dyes; 0/Reactive Nitrogen Species; 0/Reactive Oxygen Species; 0/Rhodamines; 0/aminophenyl fluorescein; 0/dihydroethidine; 632-93-9/Dicarbethoxydihydrocollidine; 76-54-0/2',7'-dichlorofluorescein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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