Document Detail

The use of ethidium bromide to assess a novel injury/recovery phenomenon in Listeria monocytogenes in inhibitory NaCl conditions.
MedLine Citation:
PMID:  9418074     Owner:  NLM     Status:  MEDLINE    
An injury and recovery phenomenon was observed in Listeria monocytogenes inoculated into a medium containing 2.2 mmol l -1 NaCl, a concentration that was inhibitory to growth. The apparent loss then recovery of viability, as determined by plate counts, was compared with the uptake of ethidium bromide by the cells and found to be inversely related. Injury was caused not only by the initial osmotic up-shock but also by the subsequent down-shock involved in the spread plate protocol.
T P Robinson; M J Ocio; F Lyn; A Kaloti; B M Mackey
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Letters in applied microbiology     Volume:  25     ISSN:  0266-8254     ISO Abbreviation:  Lett. Appl. Microbiol.     Publication Date:  1997 Nov 
Date Detail:
Created Date:  1998-01-15     Completed Date:  1998-01-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8510094     Medline TA:  Lett Appl Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  367-70     Citation Subset:  IM    
Institute of Food Research, Reading, Berkshire, UK.
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MeSH Terms
Ethidium / pharmacology*
Listeria monocytogenes / drug effects*,  growth & development
Sodium Chloride / pharmacology*
Reg. No./Substance:
3546-21-2/Ethidium; 7647-14-5/Sodium Chloride
Erratum In:
Lett Appl Microbiol 1998 Apr;26(4):332

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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