Document Detail

The use of enzyme technology for improved energy utilization from full-fat oilseeds. Part II: flaxseed.
MedLine Citation:
PMID:  16776472     Owner:  NLM     Status:  MEDLINE    
An in vitro incubation study was carried out to determine whether various carbohydrase preparations contained appropriate activities to target nonstarch polysaccharides (NSP) of full-fat flaxseed. Enzyme preparations C (cellulase, 340 U/g), XG (xylanase, 63,600 U/g and glucanase, 48,300 U/g), P (pectinase, 10,000 U/g), and MC (mannanase, 10,900 U/g and cellulase, 600 U/ g), alone and in combination (C + P, C + XG, P + XG, C + P + XG, C + P + MC, and C + P + XG + MC), were evaluated. Triplicate samples of defatted flaxseed meal (0.1 g) were incubated with 1% single enzymes or combinations at 45 degrees C and pH 5.2. A more pronounced degradation of NSP was achieved when the enzyme preparations were used in concert. Compared with the control (no enzyme) treatment, the degree of NSP degradation averaged 34.7% when the sample was incubated with the 3 most effective enzyme combinations (C + P + XG, C + P + MC, and C + P + XG + MC). The effect of carbohydrase enzyme supplementation on energy utilization from full-fat flaxseed was investigated in a TMEn assay with adult roosters. When compared with the nonsupplemented sample, an increase (P < 0.05) in TMEn content from 2,717 to 3,751 kcal/kg (on average) was observed for the flaxseed supplemented with enzymes C + P + XG, C + P + MC, and C + P + XG + MC. A similar pattern of increase (P < 0.05) in fat and NSP digestibilities was noted. Enzyme combination C + P + XG was further evaluated in a 2-wk (5- to 18-d) trial with broiler chickens fed a corn and soybean meal-based flaxseed (15%) diet or the flaxseed diet supplemented with the enzyme at 3 different levels: 0.002, 0.01, and 0.05%. When supplemented at the highest level, the enzyme blend improved (P < 0.05) feed:gain, total tract DM, fat and NSP digestibilities, AMEn content, and ileal fat digestibility. No effect of enzyme supplementation, regardless of the level used, on ileal protein digestibility and digesta viscosity was observed. The results of the current study suggest that multiactivity carbohydrase enzyme supplements may be used as a means to improve energy utilization from full-fat flaxseed and, thus, enhance its feeding value for poultry.
B A Slominski; X Meng; L D Campbell; W Guenter; O Jones
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Poultry science     Volume:  85     ISSN:  0032-5791     ISO Abbreviation:  Poult. Sci.     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-06-16     Completed Date:  2006-07-05     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0401150     Medline TA:  Poult Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1031-7     Citation Subset:  IM    
Department of Animal Science, University of Manitoba, Winnipeg, Canada.
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MeSH Terms
Animal Feed
Animal Nutritional Physiological Phenomena
Cellulase / administration & dosage
Chickens / metabolism*
Energy Metabolism*
Fats / analysis
Flax / chemistry*,  metabolism
Glycoside Hydrolases / administration & dosage*
Plant Oils / metabolism*
Polygalacturonase / administration & dosage
Polysaccharides / metabolism
Reg. No./Substance:
0/Fats; 0/Plant Oils; 0/Polysaccharides; EC 3.2.1.-/Glycoside Hydrolases; EC 3.2.1.-/carbohydrase; EC; EC

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