Document Detail

The use of embryo transfer to produce pregnancies in repeat-breeding dairy cattle.
MedLine Citation:
PMID:  17942148     Owner:  NLM     Status:  MEDLINE    
Repeat breeding is an important factor affecting economic success in dairy management. The objective of this study was to investigate the effectiveness of transfer of frozen-thawed IVF embryos in establishing pregnancy in repeat-breeding Holstein cattle. Cumulus oocyte complexes were collected by aspiration of 2-5 mm follicles from ovaries obtained at two local abattoirs. After IVF, days 7 and 8 blastocysts were frozen either in 1.5M ethylene glycol with 0.1M sucrose, or in 1.4M glycerol with 0.1M sucrose. Holstein recipients (122 heifers and 410 cows) included those that had not conceived after 3-21 inseminations. Embryos frozen in ethylene glycol were transferred directly, and embryos frozen in glycerol were transferred after dilution of the cryoprotectant in sucrose into recipients 7 or 8 days after estrus (without-AI group), or following AI (with-AI group). Pregnancy rates were compared by the Chi-square test. Significantly higher pregnancy rates were achieved by embryo transfer following AI (with-AI group) than by embryo transfer alone (without-AI group) in both heifers (49.2 and 29.5%, respectively) and cows (41.5 and 20.4%, respectively; P<0.05). Pregnancy rates were not significantly different between heifers and cows. However, pregnancy rate decreased as the number of inseminations prior to embryo transfer increased in the with-AI group, but not in the without-AI group. Therefore, transfer of frozen-thawed IVF embryos during the same cycle in which AI was done improved pregnancy rates in repeat-breeding Holstein heifers and cows, and suggested that embryo transfer is an alternative in the treatment of repeat breeding.
O Dochi; K Takahashi; T Hirai; H Hayakawa; M Tanisawa; Y Yamamoto; H Koyama
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Publication Detail:
Type:  Journal Article     Date:  2007-10-17
Journal Detail:
Title:  Theriogenology     Volume:  69     ISSN:  0093-691X     ISO Abbreviation:  Theriogenology     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2007-12-06     Completed Date:  2008-03-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  124-8     Citation Subset:  IM    
Rakuno Gakuen University, Ebetsu, Japan.
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MeSH Terms
Animals, Newborn
Cattle / embryology,  physiology*
Cryopreservation / veterinary
Cryoprotective Agents / pharmacology
Embryo Transfer / methods,  standards,  veterinary*
Fertilization in Vitro / methods,  standards,  veterinary*
Insemination, Artificial / standards,  veterinary*
Reg. No./Substance:
0/Cryoprotective Agents

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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