Document Detail


The use of doxycycline as a protectant against sulphur mustard in HaCaT cells.
MedLine Citation:
PMID:  18069744     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
As part of an ongoing programme on medical countermeasures against the chemical warfare agent sulphur mustard (HD) and set against the background of the involvement of matrix metalloproteinases (MMPs) in the pathology of HD-induced vesication processes, the potentially beneficial effects of doxycycline on cell attachment was determined in confluent HaCaT cell cultures exposed to HD. Doxycycline was found to inhibit to a significant extent the tendency of HD-exposed cells to detach from the growth substrate, however, analysis of the metabolic activity of the adherent cells indicated that doxycycline treatment did not maintain cell viability. It was confirmed that apoptosis was the predominant mode of HD-induced cell death. The results suggested that doxycycline and other MMP inhibitors may have a role to play in therapeutic intervention against HD exposure, but only as part of a combination therapy. The specific value of protease inhibitors in this capacity remains to be determined.
Authors:
Christopher D Lindsay; Edgar Gentilhomme; Jacques D Mathieu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of applied toxicology : JAT     Volume:  28     ISSN:  0260-437X     ISO Abbreviation:  J Appl Toxicol     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-06-30     Completed Date:  2008-09-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8109495     Medline TA:  J Appl Toxicol     Country:  England    
Other Details:
Languages:  eng     Pagination:  665-73     Citation Subset:  IM    
Copyright Information:
2008 John Wiley & Sons, Ltd
Affiliation:
Biomedical Sciences Department, Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, UK. cdlindsay@dstl.gov.uk
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MeSH Terms
Descriptor/Qualifier:
Anti-Bacterial Agents / pharmacology*
Cell Count
Cell Cycle / drug effects
Cell Line
Chemical Warfare Agents / toxicity*
Coloring Agents
Doxycycline / pharmacology*
Flow Cytometry
Gentian Violet
Humans
Keratinocytes / drug effects*,  metabolism,  ultrastructure
Microscopy, Phase-Contrast
Mustard Gas / toxicity*
Tetrazolium Salts
Thiazoles
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Chemical Warfare Agents; 0/Coloring Agents; 0/Tetrazolium Salts; 0/Thiazoles; 298-93-1/thiazolyl blue; 505-60-2/Mustard Gas; 548-62-9/Gentian Violet; 564-25-0/Doxycycline

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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