Document Detail

The use of cyanobacteria as filler in nitrocellulose capillaries improves ultrastructural preservation of immature barley pollen upon high pressure freezing.
MedLine Citation:
PMID:  21711458     Owner:  NLM     Status:  Publisher    
The high pressure freezing (HPF) followed by freeze substitution technique has advantages over chemical fixation in the context of preserving sample ultrastructure. However, when HPF is applied to cultured pollen grains, the large intercellular spaces present lead to a poor level of ultrastructure preservation. We report here that the mixing of cyanobacteria with immature barley pollen grains succeeded in greatly reducing the volume of liquid present between the large pollen grains, and so improved the loading of the sample into a nitrocellulose capillary. The use of yeast or cyanobacteria paste to surround the filled capillaries was beneficial in speeding the transfer of heat during the freezing process. This modification of the HPF method resulted in a greatly improved level of ultrastructure preservation.
D S Daghma; J Kumlehn; M Melzer
Related Documents :
18083468 - How often should we perform arterial blood gas analysis during thoracoscopic surgery?
8971298 - Intravenous midazolam as a sedative for colonoscopy: a randomized, double-blind clinica...
21848288 - Polyamorphic amorphous silicon at high pressure: raman and spatially resolved x-ray sca...
16869528 - Does hydrostatic pressure have an effect on reactive oxygen species in the eel?
4006648 - Continuous recording of pyloric sphincter pressure in dogs. relationship to migratory m...
24775098 - Lower potassium intake is associated with increased wave reflection in young healthy ad...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-6-29
Journal Detail:
Title:  Journal of microscopy     Volume:  -     ISSN:  1365-2818     ISO Abbreviation:  -     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-6-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0204522     Medline TA:  J Microsc     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2011 The Authors Journal of Microscopy © 2011 Royal Microscopical Society.
Department of Physiology and Cell Biology, Leibniz Institute of Plant Genetics and Crop Plant Research, D-06466 Gatersleben, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Assessment of specific characteristics of abnormal general movements: does it enhance the prediction...
Next Document:  Smad8 is expressed in the anterior necrotic zone: Evidence for a role of bone morphogenetic proteins...