Document Detail


The use of chitosan to increase the stability of calcium alginate beads with entrapped yeast cells.
MedLine Citation:
PMID:  8679112     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method was developed for increasing the stability of calcium alginate beads with entrapped Saccharomyces cerevisiae cells. Polyelectrolyte complexes were used as surface-coating materials by dropping alginate into chitosan. The coacervation agent sodium triphosphate was needed for the formation of the polyelectrolyte complexes. Gel beads maintained their rigidity during ethanol production and were chemically stable in phosphate buffer. No differences in ethanol production were found between S. cerevisiae immobilized in calcium alginate and in calcium alginate coated with polyelectrolyte complexes. The maximum productivity by immobilized S. cerevisiae cells was 74.1 g of ethanol/h per g of gel beads obtained at pH 4.8-6.0 and at 30 degrees C.
Authors:
X Li
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnology and applied biochemistry     Volume:  23 ( Pt 3)     ISSN:  0885-4513     ISO Abbreviation:  Biotechnol. Appl. Biochem.     Publication Date:  1996 Jun 
Date Detail:
Created Date:  1996-08-22     Completed Date:  1996-08-22     Revised Date:  2007-03-21    
Medline Journal Info:
Nlm Unique ID:  8609465     Medline TA:  Biotechnol Appl Biochem     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  269-72     Citation Subset:  IM    
Affiliation:
Department of Food Technology, Dalian College of Light Industry, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Alginates / chemistry,  metabolism*
Biocompatible Materials / chemistry,  metabolism*
Cell Compartmentation
Chitin / analogs & derivatives*,  chemistry,  metabolism
Chitosan
Drug Stability
Ethanol / metabolism
Fermentation
Gels
Glucuronic Acid
Hexuronic Acids
Hydrogen-Ion Concentration
Microspheres
Saccharomyces cerevisiae / metabolism*
Temperature
Chemical
Reg. No./Substance:
0/Alginates; 0/Biocompatible Materials; 0/Gels; 0/Hexuronic Acids; 1398-61-4/Chitin; 576-37-4/Glucuronic Acid; 64-17-5/Ethanol; 9005-32-7/alginic acid; 9012-76-4/Chitosan

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