| The use of cell division cycle mutants to investigate the control of microtubule distribution in the fission yeast Schizosaccharomyces pombe. | |
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MedLine Citation:
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PMID: 3198697 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have characterized the changes in microtubule organization that occur through the cell division cycle of the fission yeast Schizosaccharomyces pombe by indirect immunofluorescence microscopy. During interphase, groups of cytoplasmic microtubules, independent of the spindle pole body (SPB), form an array extending between the cell tips. These microtubules are involved in positioning the nucleus at the cell equator and in the establishment of cell polarity. At mitosis, the interphase array disappears and is replaced by an intranuclear spindle extending between the now duplicated SPBs. Elongation of the spindle sees the appearance of astral microtubules emanating from the cytoplasmic face of the SPBs. These persist until the end of anaphase whereupon the spindle microtubules depolymerize and two microtubule organizing centres (MTOCs) at the cell equator re-establish the interphase array. We have used the unique properties of various cell division cycle mutants to investigate further the function of these different microtubule arrays and their temporal and positional control. |
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Authors:
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I M Hagan; J S Hyams |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of cell science Volume: 89 ( Pt 3) ISSN: 0021-9533 ISO Abbreviation: J. Cell. Sci. Publication Date: 1988 Mar |
Date Detail:
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Created Date: 1989-01-26 Completed Date: 1989-01-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0052457 Medline TA: J Cell Sci Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 343-57 Citation Subset: IM |
Affiliation:
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Department of Biology, University College London, UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Cycle* Cell Division Microscopy, Fluorescence Microtubules / physiology*, ultrastructure Mutation Schizosaccharomyces |
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