Document Detail

The use of capillary electrophoresis for point-mutation screening.
MedLine Citation:
PMID:  9369028     Owner:  NLM     Status:  MEDLINE    
Advances in capillary electrophoresis technology over the past three years have been rapid. Capillary electrophoresis offers high-throughput, high-resolution, automatic operation and on-line detection with automatic data acquisition, and this has stimulated its application to the analysis of DNA mutations. Many different PCR-based DNA-mutation assays have been developed for unknown and known mutations. This article compares conventional PCR-based mutation-detection assays with the methods developed for use with capillary electrophoresis. Future trends for mutation detection using capillary electrophoresis are also assessed, with a special emphasis on totally integrated, microchip capillary-electrophoresis-based mutation-detection systems.
K R Mitchelson; J Cheng; L J Kricka
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Trends in biotechnology     Volume:  15     ISSN:  0167-7799     ISO Abbreviation:  Trends Biotechnol.     Publication Date:  1997 Nov 
Date Detail:
Created Date:  1997-12-04     Completed Date:  1997-12-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8310903     Medline TA:  Trends Biotechnol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  448-58     Citation Subset:  B    
ForBio Research Pty Ltd, Indooroopilly, Australia.
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MeSH Terms
Electrophoresis, Capillary / methods*
Point Mutation*
Polymerase Chain Reaction
Polymorphism, Genetic
Reg. No./Substance:

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