Document Detail


On the use of Zn2+ to discriminate endonucleases activated during apoptosis.
MedLine Citation:
PMID:  9352093     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
One approach to discriminate among specific DNases in apoptosis is to use inhibitors specific for each endonuclease. Zn2+ is known to inhibit Ca(2+)- and Mg(2+)-dependent endonuclease enzymatic activities during apoptosis. Acidic DNases were thought to be insensitive to Zn2+. In this paper, we analyse the effects of Zn2+ on activity of DNase II, either purified or in nuclei from lens fiber cells. These cells follow a physiological nuclear degeneration with DNase II accumulation in their nuclei. We show that Zn2+ is able to inhibit also this acidic endonuclease at a concentration of 1-6 mM. At a higher concentration of Zn2+, DNA is extensively degraded during the assay, masking the inhibition of the enzyme. This DNA degradation in the presence of Zn2+ has led to an overestimation of the activity of DNase II in studies of apoptosis. Hence, Zn2+ cannot be used to specifically identify one endonuclease among the different DNases involved in nuclear degradation during programmed cell death.
Authors:
A Torriglia; E Chaudun; Y Courtois; M F Counis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimie     Volume:  79     ISSN:  0300-9084     ISO Abbreviation:  Biochimie     Publication Date:  1997 Jul 
Date Detail:
Created Date:  1997-12-04     Completed Date:  1997-12-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1264604     Medline TA:  Biochimie     Country:  FRANCE    
Other Details:
Languages:  eng     Pagination:  435-8     Citation Subset:  IM    
Affiliation:
Développement, pathologie et vieillissement de la rétine, U450 INSERM-CNRS, Association Claude Bernard, Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis* / drug effects
Cattle
Chick Embryo
DNA Fragmentation / drug effects
Endodeoxyribonucleases / antagonists & inhibitors,  drug effects*,  metabolism*
Enzyme Activation / drug effects
Escherichia coli
Lens Cortex, Crystalline / cytology,  drug effects,  enzymology
Swine
Zinc / pharmacology*
Chemical
Reg. No./Substance:
7440-66-6/Zinc; EC 3.1.-/Endodeoxyribonucleases; EC 3.1.21.-/calcium magnesium dependent endodeoxyribonuclease; EC 3.1.22.1/deoxyribonuclease II

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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