| On the use of Zn2+ to discriminate endonucleases activated during apoptosis. | |
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MedLine Citation:
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PMID: 9352093 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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One approach to discriminate among specific DNases in apoptosis is to use inhibitors specific for each endonuclease. Zn2+ is known to inhibit Ca(2+)- and Mg(2+)-dependent endonuclease enzymatic activities during apoptosis. Acidic DNases were thought to be insensitive to Zn2+. In this paper, we analyse the effects of Zn2+ on activity of DNase II, either purified or in nuclei from lens fiber cells. These cells follow a physiological nuclear degeneration with DNase II accumulation in their nuclei. We show that Zn2+ is able to inhibit also this acidic endonuclease at a concentration of 1-6 mM. At a higher concentration of Zn2+, DNA is extensively degraded during the assay, masking the inhibition of the enzyme. This DNA degradation in the presence of Zn2+ has led to an overestimation of the activity of DNase II in studies of apoptosis. Hence, Zn2+ cannot be used to specifically identify one endonuclease among the different DNases involved in nuclear degradation during programmed cell death. |
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Authors:
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A Torriglia; E Chaudun; Y Courtois; M F Counis |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochimie Volume: 79 ISSN: 0300-9084 ISO Abbreviation: Biochimie Publication Date: 1997 Jul |
Date Detail:
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Created Date: 1997-12-04 Completed Date: 1997-12-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1264604 Medline TA: Biochimie Country: FRANCE |
Other Details:
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Languages: eng Pagination: 435-8 Citation Subset: IM |
Affiliation:
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Développement, pathologie et vieillissement de la rétine, U450 INSERM-CNRS, Association Claude Bernard, Paris, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis* / drug effects Cattle Chick Embryo DNA Fragmentation / drug effects Endodeoxyribonucleases / antagonists & inhibitors, drug effects*, metabolism* Enzyme Activation / drug effects Escherichia coli Lens Cortex, Crystalline / cytology, drug effects, enzymology Swine Zinc / pharmacology* |
| Chemical | |
Reg. No./Substance:
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7440-66-6/Zinc; EC 3.1.-/Endodeoxyribonucleases; EC 3.1.21.-/calcium magnesium dependent endodeoxyribonuclease; EC 3.1.22.1/deoxyribonuclease II |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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