Document Detail


The use of Mammalian cultured cells loaded with a fluorescent dye shows specific membrane penetration of undissociated acetic Acid.
MedLine Citation:
PMID:  22451395     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Acetic acid induces unique physiological responses in mammalian cells. Our previous study found that fura-2-loaded human embryonic kidney (HEK) 293T cells showed a robust intracellular fluorescence response immediately after stimulation with acetic acid, and no such response in the case of citric acid. In the present study, we aimed to identify the unique characteristics of acetic acid responsible for this phenomenon. We found that one such feature is its hydrophobicity. We also discovered that acetic acid induces cell responses by intracellular acidification. Of the components of acetic acid in solution (protons, acetate ions, and undissociated acetic acid), undissociated acetic acid might be the functional unit that penetrates the lipid bilayer of cell membranes to acidify the intracellular environment, thereby inducing cell responses. The method used in this study might be convenient in evaluating the intracellular acidification of cultured cells by acids in the external environment.
Authors:
Sho Ishii; Mikiya Kishi; Keigo Yamagami; Shinji Okada; Keiko Abe; Takumi Misaka
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  76     ISSN:  1347-6947     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2012  
Date Detail:
Created Date:  2012-03-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  523-9     Citation Subset:  IM    
Affiliation:
Central Research Institute, Mizkan Group Co.
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