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The use of DNA fingerprinting to resolve conflicting results in patients with suspected gastrointestinal malignancy.
MedLine Citation:
PMID:  23391868     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
GOALS: : To underscore the utility of DNA fingerprinting for clarifying disparate results from endoscopic pathologic specimens.
BACKGROUND: : Occasionally, serially obtained gastrointestinal biopsies may yield inconsistent results. These discrepancies pose a dilemma for gastroenterologists and their patients, especially when malignancy is a consideration.
STUDY: : Patients referred to our tertiary care center from outside institutions had undergone endoscopically obtained esophageal biopsies showing malignancy, verified by pathologists at both our site and from the referring center. Repeat endoscopic biopsies at our center did not show malignancy. To verify that different sets of biopsies came from the same patient, we performed a polymerase chain reaction-based analysis comparing the 2 specimens. This analysis, called DNA fingerprinting, can show a high degree of certainty whether 2 specimens came from the same patient.
RESULTS: : In each case, DNA fingerprinting verified a match, laying the groundwork for intervention. One patient underwent endoscopic radiofrequency ablation to the esophageal mucosa involved. Another underwent esophagectomy with partial gastrectomy. Both are doing well clinically and remain cancer-free on follow-up.
CONCLUSIONS: : DNA fingerprinting is a powerful and a relatively inexpensive tool. Usually, only small amounts of tissue are required, and even degraded or archival tissue is adequate. DNA fingerprinting can be an important tool in the gastroenterologist's arsenal to help clarify conflicting results, allowing the patient and physician to move forward with the management.
Authors:
Sameer Islam; Ethan D Miller; Neal Patel; Giovanni De Petris; Edward W Highsmith; David E Fleischer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of clinical gastroenterology     Volume:  47     ISSN:  1539-2031     ISO Abbreviation:  J. Clin. Gastroenterol.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7910017     Medline TA:  J Clin Gastroenterol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  239-41     Citation Subset:  IM    
Affiliation:
*Division of Gastroenterology, Mayo Clinic-Gastroenterology †Department of Laboratory Medicine & Pathology, Mayo Clinic, Scottsdale, AZ ‡Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN.
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