Document Detail

The use of DNA fingerprinting to resolve conflicting results in patients with suspected gastrointestinal malignancy.
MedLine Citation:
PMID:  23391868     Owner:  NLM     Status:  In-Data-Review    
GOALS: : To underscore the utility of DNA fingerprinting for clarifying disparate results from endoscopic pathologic specimens.
BACKGROUND: : Occasionally, serially obtained gastrointestinal biopsies may yield inconsistent results. These discrepancies pose a dilemma for gastroenterologists and their patients, especially when malignancy is a consideration.
STUDY: : Patients referred to our tertiary care center from outside institutions had undergone endoscopically obtained esophageal biopsies showing malignancy, verified by pathologists at both our site and from the referring center. Repeat endoscopic biopsies at our center did not show malignancy. To verify that different sets of biopsies came from the same patient, we performed a polymerase chain reaction-based analysis comparing the 2 specimens. This analysis, called DNA fingerprinting, can show a high degree of certainty whether 2 specimens came from the same patient.
RESULTS: : In each case, DNA fingerprinting verified a match, laying the groundwork for intervention. One patient underwent endoscopic radiofrequency ablation to the esophageal mucosa involved. Another underwent esophagectomy with partial gastrectomy. Both are doing well clinically and remain cancer-free on follow-up.
CONCLUSIONS: : DNA fingerprinting is a powerful and a relatively inexpensive tool. Usually, only small amounts of tissue are required, and even degraded or archival tissue is adequate. DNA fingerprinting can be an important tool in the gastroenterologist's arsenal to help clarify conflicting results, allowing the patient and physician to move forward with the management.
Sameer Islam; Ethan D Miller; Neal Patel; Giovanni De Petris; Edward W Highsmith; David E Fleischer
Related Documents :
14531698 - Nanocrystals modified with peptide nucleic acids (pnas) for selective self-assembly and...
19823748 - Arginine-based pna microarrays for apoe genotyping.
18776348 - Ion-exchange capture of labeled pyrrolidinyl peptide nucleic acids for dna sequence det...
7958978 - Sequence-specific transcription arrest by peptide nucleic acid bound to the dna templat...
15173578 - Polyadenylation of rrna in saccharomyces cerevisiae.
7519728 - Induction of tandem-base change mutations.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of clinical gastroenterology     Volume:  47     ISSN:  1539-2031     ISO Abbreviation:  J. Clin. Gastroenterol.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7910017     Medline TA:  J Clin Gastroenterol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  239-41     Citation Subset:  IM    
*Division of Gastroenterology, Mayo Clinic-Gastroenterology †Department of Laboratory Medicine & Pathology, Mayo Clinic, Scottsdale, AZ ‡Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Does increased gamma activity in patients suffering from Parkinson' disease counteract the movement ...
Next Document:  The severity of histologic liver lesions is independent of body mass index in patients with nonalcoh...