| The use of the 2-aminobenzoic acid tag for oligosaccharide gel electrophoresis. | |
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MedLine Citation:
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PMID: 11005578 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Gel electrophoresis of fluorophore labeled saccharides provides a rapid and reliable method to screen enzymatic and/or chemical treatments of polysaccharides and glycoconjugates, as well as a sensitive and efficient microscale method to separate and purify oligosaccharides for further analysis. A simple and inexpensive method of derivatization and analysis using 2-aminobenzoic acid (anthranilic acid, AA) is described and applied to the extracellular polysaccharide released by the desiccation tolerant cyanobacterium Nostoc commune DRH-1. The results of these analyses suggest a possible protective functionality of two pendent groups, as well as a potential relationship between these groups and the desiccation tolerance of the organism. |
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Authors:
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Z Huang; T Prickett; M Potts; R F Helm |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Carbohydrate research Volume: 328 ISSN: 0008-6215 ISO Abbreviation: Carbohydr. Res. Publication Date: 2000 Aug |
Date Detail:
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Created Date: 2001-02-02 Completed Date: 2001-02-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0043535 Medline TA: Carbohydr Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 77-83 Citation Subset: IM |
Affiliation:
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Fralin Biotechnology Center, Virginia Tech, Blacksburg 24061, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anthranilic Acids
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chemistry* Carbohydrate Sequence Cyanobacteria / chemistry* Electrophoresis, Polyacrylamide Gel / methods* Fluorescent Dyes Molecular Sequence Data Oligosaccharides / isolation & purification* Polysaccharides, Bacterial / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Anthranilic Acids; 0/Fluorescent Dyes; 0/Oligosaccharides; 0/Polysaccharides, Bacterial; 118-92-3/anthranilic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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