Document Detail


The use of the 2-aminobenzoic acid tag for oligosaccharide gel electrophoresis.
MedLine Citation:
PMID:  11005578     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Gel electrophoresis of fluorophore labeled saccharides provides a rapid and reliable method to screen enzymatic and/or chemical treatments of polysaccharides and glycoconjugates, as well as a sensitive and efficient microscale method to separate and purify oligosaccharides for further analysis. A simple and inexpensive method of derivatization and analysis using 2-aminobenzoic acid (anthranilic acid, AA) is described and applied to the extracellular polysaccharide released by the desiccation tolerant cyanobacterium Nostoc commune DRH-1. The results of these analyses suggest a possible protective functionality of two pendent groups, as well as a potential relationship between these groups and the desiccation tolerance of the organism.
Authors:
Z Huang; T Prickett; M Potts; R F Helm
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Carbohydrate research     Volume:  328     ISSN:  0008-6215     ISO Abbreviation:  Carbohydr. Res.     Publication Date:  2000 Aug 
Date Detail:
Created Date:  2001-02-02     Completed Date:  2001-02-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0043535     Medline TA:  Carbohydr Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  77-83     Citation Subset:  IM    
Affiliation:
Fralin Biotechnology Center, Virginia Tech, Blacksburg 24061, USA.
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MeSH Terms
Descriptor/Qualifier:
Anthranilic Acids / chemistry*
Carbohydrate Sequence
Cyanobacteria / chemistry*
Electrophoresis, Polyacrylamide Gel / methods*
Fluorescent Dyes
Molecular Sequence Data
Oligosaccharides / isolation & purification*
Polysaccharides, Bacterial / chemistry*
Chemical
Reg. No./Substance:
0/Anthranilic Acids; 0/Fluorescent Dyes; 0/Oligosaccharides; 0/Polysaccharides, Bacterial; 118-92-3/anthranilic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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