Document Detail

The use of the [1,2-13C]acetate recovery factor in metabolic research.
MedLine Citation:
PMID:  12682837     Owner:  NLM     Status:  MEDLINE    
To provide guidelines on the correct application of the acetate recovery factor in metabolic research, we investigated the influence of exercise intensity and infusion protocol on [1,2-(13)C]acetate label recovery during exercise. Eight cyclists were studied during [1,2-(13)C]acetate infusion for 1 h at rest followed by three 30-min stages of cycling exercise at a workload of 40, 55 and 75% maximal workload ( W(max)), respectively (protocol 1). Four cyclists were subsequently studied following [1,2-(13)C]acetate infusion in three separate trials while cycling at the same workloads but in the absence of any pre-exercise infusion period (protocol 2). Finally, we observed the cyclists during [1,2-(13)C]acetate infusion at a 40% W(max) workload after 4 h of pre-exercise infusion (protocol 3). Acetate recovery increased from 13.7 (0.4)%, after 1 h of rest, to a plateau value of 75.1 (2), 91.2 (0.7) and 101 (2)% during exercise at 40, 55 and 75% W(max) workloads, respectively. In protocol 2, without prior infusion time, fractional label recovery was substantially lower at each separate workload. In contrast, when applying an extensive pre-exercise infusion period of 4 h, acetate recovery rates were substantially increased compared to the values observed in protocols 1 and 2 during exercise at a 40% W(max) workload. In conclusion, in contrast to resting conditions, acetate recovery reaches a plateau value during exercise. Though this plateau value is repeatedly used to correct for label recovery in various exercise studies, our data clearly show that acetate label recovery during exercise not only depends on the exercise intensity but also on the applied infusion protocol. Therefore, theoretical acetate recovery factors taken from previous literature are not generally applicable.
Luc J C van Loon; Rene Koopman; Patrick Schrauwen; Jos Stegen; Anton J M Wagenmakers
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Publication Detail:
Type:  Clinical Conference; Comparative Study; Journal Article     Date:  2003-04-01
Journal Detail:
Title:  European journal of applied physiology     Volume:  89     ISSN:  1439-6319     ISO Abbreviation:  Eur. J. Appl. Physiol.     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-05-08     Completed Date:  2004-02-27     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  100954790     Medline TA:  Eur J Appl Physiol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  377-83     Citation Subset:  IM    
Nutrition Research Institute Maastricht (NUTRIM), Department of Human Biology, Maastricht University, PO Box 616, 6200 MD Maastricht, The Netherlands.
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MeSH Terms
Acetates / administration & dosage*,  pharmacokinetics*
Carbon Dioxide / metabolism*
Carbon Isotopes / pharmacokinetics
Energy Metabolism / physiology
Exercise / physiology
Infusions, Intravenous / methods*
Metabolic Clearance Rate / physiology
Oxygen Consumption / physiology
Physical Exertion / physiology*
Pulmonary Gas Exchange / physiology
Radioisotope Dilution Technique*
Radiopharmaceuticals / pharmacokinetics
Research Design
Reg. No./Substance:
0/Acetates; 0/Carbon Isotopes; 0/Radiopharmaceuticals; 124-38-9/Carbon Dioxide

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