Document Detail


The urinary podocyte as a marker for the differential diagnosis of idiopathic focal glomerulosclerosis and minimal-change nephrotic syndrome.
MedLine Citation:
PMID:  10878397     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND/AIMS: Detection of podocytes in the urine sediment of children indicates that severe podocyte injury occurred in the glomerulus. Focal glomerulosclerosis (FGS) and minimal-change nephrotic syndrome (MCNS) are kidney diseases characterized by massive proteinuria. The aim of the present study was to determine whether urinary podocytes can be detected in patients with idiopathic FGS or MCNS and whether immunosuppression therapy alters these cells. METHODS: Twenty patients with MCNS (nephrotic stage, n = 12; remission stage, n = 8), 15 patients with FGS and 20 healthy controls were included in the present study. Urinary podocytes were stained by immunofluorescence. All patients with MCNS at the nephrotic stage received prednisolone for 6 months, and all patients with FGS received some form of immunosuppression therapy including prednisolone, cyclophosphamide or mizoribine for 12 months. RESULTS: The 12 nephrotic-stage MCNS patients achieved remission after treatment. Seven of the 15 FGS patients also achieved remission, but the other 8 remained in the nephrotic stage. Urinary podocytes were not detected in any patient with MCNS nor were they detected in healthy controls. Urinary podocytes were detected in all FGS patients (mean, 4.2 cells/ml) before treatment and the number of cells decreased in the 7 patients who achieved remission. The number of podocytes was unchanged in the other 8 patients even after treatment. CONCLUSION: Urinary podocytes may be a useful diagnostic indicator for differentiation between FGS and MCNS. These cells may also mark disease progression in cases of FGS.
Authors:
T Nakamura; C Ushiyama; S Suzuki; M Hara; N Shimada; I Ebihara; H Koide
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  American journal of nephrology     Volume:  20     ISSN:  0250-8095     ISO Abbreviation:  Am. J. Nephrol.     Publication Date:    2000 May-Jun
Date Detail:
Created Date:  2000-09-19     Completed Date:  2000-09-19     Revised Date:  2007-02-14    
Medline Journal Info:
Nlm Unique ID:  8109361     Medline TA:  Am J Nephrol     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  175-9     Citation Subset:  IM    
Copyright Information:
Copyright 2000 S. Karger AG, Basel
Affiliation:
Department of Medicine, Misato Junshin Hospital, Saitama, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adult
Anti-Inflammatory Agents / therapeutic use
Biological Markers / urine
Case-Control Studies
Cyclophosphamide / therapeutic use
Diagnosis, Differential
Disease Progression
Female
Fluorescent Antibody Technique
Glomerulosclerosis, Focal Segmental / diagnosis*,  drug therapy,  urine
Humans
Immunosuppressive Agents / therapeutic use
Male
Nephrosis, Lipoid / diagnosis*,  drug therapy,  urine
Prednisolone / therapeutic use
Proteinuria
Ribonucleosides / therapeutic use
Statistics, Nonparametric
Treatment Outcome
Urine / cytology*
Chemical
Reg. No./Substance:
0/Anti-Inflammatory Agents; 0/Biological Markers; 0/Immunosuppressive Agents; 0/Ribonucleosides; 50-18-0/Cyclophosphamide; 50-24-8/Prednisolone; 50924-49-7/bredinin

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