| The urinary excretion of orotic acid and orotidine, measured by an isotope dilution assay. | |
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MedLine Citation:
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PMID: 365397 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Unknown concentrations of orotic acid can be measured by competition with a known amount of [carboxyl-14C]orotic acid for reaction with a limiting amount of phosphoribosylpyrophosphate in the presence of orotate phosphoribosyltransferase and orotidine monophosphate decarboxylase. The dilution of the specific radioactivity in the product 14CO2 is a sensitive and accurate measure of the amount of orotic acid present in the sample. Orotidine can also be determined after hydrolytic cleavage to orotic acid. The method was used to measure orotic acid and orotidine in urine samples from newborns, healthy controls and patients with gout or deficiency of hypoxanthine-guanine phosphoribosyltransferase receiving allopurinol. Urinary excretion of orotic acid and orotidine in newborns was similar whether the infants were breast-fed or received milk powder. The excretion of orotidine was increased in all patients receiving allopurinol. After allopurinol administration orotic acid excretion was increased in gouty patients but close to normal values in patients with deficiency of hypoxanthine-guanine phosphoribosyltransferase. The results are discussed in relation to the mechanism by which allopurinol inhibits pyrimidine metabolism. |
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Authors:
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W J Tax; J H Veerkamp; E D Schretlen |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Clinica chimica acta; international journal of clinical chemistry Volume: 90 ISSN: 0009-8981 ISO Abbreviation: Clin. Chim. Acta Publication Date: 1978 Dec |
Date Detail:
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Created Date: 1979-03-24 Completed Date: 1979-03-24 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 1302422 Medline TA: Clin Chim Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 217-23 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Aging Allopurinol / therapeutic use* Breast Feeding Child Female Gout / drug therapy, urine* Humans Hypoxanthine Phosphoribosyltransferase / deficiency* Infant, Newborn Orotate Phosphoribosyltransferase Orotic Acid / urine* Phosphoribosyl Pyrophosphate Pregnancy Radioisotope Dilution Technique Uridine / analogs & derivatives*, urine |
| Chemical | |
Reg. No./Substance:
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315-30-0/Allopurinol; 58-96-8/Uridine; 65-86-1/Orotic Acid; 7540-64-9/Phosphoribosyl Pyrophosphate; EC 2.4.2.10/Orotate Phosphoribosyltransferase; EC 2.4.2.8/Hypoxanthine Phosphoribosyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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