Document Detail

The urea cycle of Helicobacter pylori.
MedLine Citation:
PMID:  8885413     Owner:  NLM     Status:  MEDLINE    
The presence and activities of the enzymes of the urea cycle in the bacterium Helicobacter pylori were investigated employing one- and two-dimensional NMR spectroscopy and radioactive tracer analysis. Cell suspensions, lysates and membrane preparations generated L-ornithine and ammonium at high rates in incubations with L-arginine, indicating the presence of arginase activity. Anabolic ornithine transcarbamoylase (OTCase) activity was identified by the formation of heat-stable products in incubations of cell-free extracts with ornithine and radiolabelled carbamoyl phosphate. The heat-labile product that resulted from incubations of cell-free extracts with citrulline radiolabelled in the guanidino moiety revealed the presence of catabolic OTCase activity. Argininosuccinate formation and catalysis indicated the presence of argininosuccinate synthetase and argininosuccinase activities. The findings suggested that H. pylori has a urea cycle which acts as an effective mechanism to extrude excess nitrogen from cells.
G L Mendz; S L Hazell
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  142 ( Pt 10)     ISSN:  1350-0872     ISO Abbreviation:  Microbiology (Reading, Engl.)     Publication Date:  1996 Oct 
Date Detail:
Created Date:  1997-01-23     Completed Date:  1997-01-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2959-67     Citation Subset:  IM    
School of Biochemistry, University of New South Wales, Sydney, Australia. G.Mendz@unsw.EDU.AU
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MeSH Terms
Arginine / metabolism
Argininosuccinate Lyase / metabolism
Argininosuccinate Synthase / metabolism
Argininosuccinic Acid / metabolism
Carbamyl Phosphate / metabolism
Helicobacter pylori / enzymology,  metabolism*
Nitrogen / metabolism
Ornithine / biosynthesis
Ornithine Carbamoyltransferase / metabolism
Time Factors
Urea / metabolism*
Reg. No./Substance:
2387-71-5/Argininosuccinic Acid; 57-13-6/Urea; 590-55-6/Carbamyl Phosphate; 7006-33-9/Ornithine; 74-79-3/Arginine; 7727-37-9/Nitrogen; EC Carbamoyltransferase; EC Lyase; EC Synthase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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