| The uptake of gamma-aminobutyrate by organotypic cultures of chick spinal cord. | |
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MedLine Citation:
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PMID: 4737378 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. Explants of spinal cord from 10-day chick embryos maintained for up to 16 days in culture rapidly accumulated gamma-amino[(3)H]butyrate when incubated at 25 degrees C or 36 degrees C in a medium containing 50nm-gamma-aminobutyrate. The mechanism of the uptake process has many of the properties of an active-transport system: it is Na(+)-dependent, temperature-sensitive, inhibited by ouabain, and displays saturation kinetics. The apparent K(m) for gamma-aminobutyrate is 1.7x10(-5)m, and V(max.) is 33pmol/min per g. 2. The rate of accumulation of gamma-amino[(3)H]butyrate in cultures between the ages of 3 and 16 days was remarkably constant and was not related to the morphological maturity of the spinal-cord explants. 3. The present demonstration in spinal-cord explants of an active transport system for gamma-aminobutyrate, already established for non-cultured nervous tissue, means that nervous-tissue culture can provide a convenient model for studying uptake processes in the central nervous system. |
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Authors:
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G Tunnicliff; Y D Cho; N Blackwell; R O Martin; J D Wood |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Biochemical journal Volume: 134 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1973 May |
Date Detail:
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Created Date: 1973-10-10 Completed Date: 1973-10-10 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 27-32 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aminobutyric Acids
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metabolism* Animals Biological Transport, Active* Chick Embryo Kinetics Organ Culture Techniques Ouabain / pharmacology Oxygen Pressure Sodium / pharmacology Spinal Cord / anatomy & histology, drug effects, metabolism* Temperature Time Factors Tritium |
| Chemical | |
Reg. No./Substance:
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0/Aminobutyric Acids; 10028-17-8/Tritium; 630-60-4/Ouabain; 7440-23-5/Sodium; 7782-44-7/Oxygen |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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