Document Detail

The uptake of gamma-aminobutyrate by organotypic cultures of chick spinal cord.
MedLine Citation:
PMID:  4737378     Owner:  NLM     Status:  MEDLINE    
1. Explants of spinal cord from 10-day chick embryos maintained for up to 16 days in culture rapidly accumulated gamma-amino[(3)H]butyrate when incubated at 25 degrees C or 36 degrees C in a medium containing 50nm-gamma-aminobutyrate. The mechanism of the uptake process has many of the properties of an active-transport system: it is Na(+)-dependent, temperature-sensitive, inhibited by ouabain, and displays saturation kinetics. The apparent K(m) for gamma-aminobutyrate is 1.7x10(-5)m, and V(max.) is 33pmol/min per g. 2. The rate of accumulation of gamma-amino[(3)H]butyrate in cultures between the ages of 3 and 16 days was remarkably constant and was not related to the morphological maturity of the spinal-cord explants. 3. The present demonstration in spinal-cord explants of an active transport system for gamma-aminobutyrate, already established for non-cultured nervous tissue, means that nervous-tissue culture can provide a convenient model for studying uptake processes in the central nervous system.
G Tunnicliff; Y D Cho; N Blackwell; R O Martin; J D Wood
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  134     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1973 May 
Date Detail:
Created Date:  1973-10-10     Completed Date:  1973-10-10     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  27-32     Citation Subset:  IM    
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MeSH Terms
Aminobutyric Acids / metabolism*
Biological Transport, Active*
Chick Embryo
Organ Culture Techniques
Ouabain / pharmacology
Sodium / pharmacology
Spinal Cord / anatomy & histology,  drug effects,  metabolism*
Time Factors
Reg. No./Substance:
0/Aminobutyric Acids; 10028-17-8/Tritium; 630-60-4/Ouabain; 7440-23-5/Sodium; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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