Document Detail


The upregulation of expressed proteins in HepG2 cells transfected by the recombinant plasmid-containing HBx gene.
MedLine Citation:
PMID:  17309779     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It is known that the hepatitis B virus X protein (HBx) plays a crucial role in the pathogenesis of HCC, but the exact functions and molecular mechanisms of HBx in HCC are not well understood. In the present study, HepG2 cell lines were cultured and transfected with pEGFP-N1 and pEGFP-N1-X. Twenty-four hours after transfection, cells were harvested and total RNA was extracted using TRIzol reagent. The expression of HBx in HepG2 cell line was assayed by real-time polymerase chain reaction and was detected by Western blotting. Moreover, proteomic analysis was performed for the HepG2-pEGFP-X cells and HepG2-pEGFP control cells. The combination of 2DE and MALDI-TOF-MS/MS revealed that SEC13L1 (SEC13-like 1 isoform b), PA28 alpha (proteasome activator REG alpha), serine-threonine kinase receptor-associated protein (STRAP) and nm23/nucleoside diphosphate kinase (NME) were upregulated in HepG2-pEGFP-X cells. STRAP is known to be a WD40 domain-containing protein, which interacts with TbetaR-I and TbetaR-II and negatively regulates TGF-beta signalling, was also found increased in human cancers. NME is known to be involved in the regulation of cancer cell progression and metastasis. These results would help the understanding of how HBx maintains tumorigenicity and progression of HCC.
Authors:
Y He; F Yang; F Wang; S-X Song; D-A Li; Y-J Guo; S-H Sun
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Scandinavian journal of immunology     Volume:  65     ISSN:  0300-9475     ISO Abbreviation:  Scand. J. Immunol.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-02-20     Completed Date:  2007-05-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0323767     Medline TA:  Scand J Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  249-56     Citation Subset:  IM    
Affiliation:
Department of Medical Genetics, Second Military Medical University, Shanghai, China.
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MeSH Terms
Descriptor/Qualifier:
Blotting, Western
Carcinoma, Hepatocellular / virology*
Carrier Proteins / biosynthesis
Cell Line
Gene Expression
Green Fluorescent Proteins
Humans
Liver Neoplasms / virology*
Microscopy, Confocal
Microscopy, Fluorescence
Muscle Proteins / biosynthesis
Nucleoside-Diphosphate Kinase / biosynthesis
Plasmids
Proteasome Endopeptidase Complex / biosynthesis
Proteins / metabolism*
Proteomics
Reverse Transcriptase Polymerase Chain Reaction
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Trans-Activators / biosynthesis*
Transfection
Up-Regulation
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Muscle Proteins; 0/Proteins; 0/Strap protein; 0/Trans-Activators; 0/enhanced green fluorescent protein; 0/hepatitis B virus X protein; 147336-22-9/Green Fluorescent Proteins; EC 2.7.4.6/Nucleoside-Diphosphate Kinase; EC 3.4.25.1/Proteasome Endopeptidase Complex

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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