Document Detail


An unusual structure at one end of potato potyvirus particles.
MedLine Citation:
PMID:  16414068     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The particles of potato virus Y (PVY) and potato virus A (PVA) potyviruses are helically constructed filaments that are thought to contain a single type of coat protein subunit. Examination of negatively stained virions by electron microscopy reveals flexuous rod-shaped particles with no obvious terminal structures. It is known that some helically constructed rod-shaped virus particles incorporate additional minor protein components, which form stable complexes that mediate particle disassembly, movement or transmission by vectors. Some of this information has been obtained using imaging techniques such as atomic force microscopy. The particles of PVY and PVA were examined by atomic force microscopy and immunogold labelling electron microscopy. Our results show that some of the potyvirus particles contain a protruding tip at one end of the virus particles, which is presumably associated with the 5'-end of viral RNA. The tip contains the virus-encoded proteins genome-linked protein and helper-component proteinase. The composition and possible roles of the terminal tip structures in virus biology are discussed.
Authors:
Lesley Torrance; Igor A Andreev; Rasa Gabrenaite-Verhovskaya; Graham Cowan; Kristiina Mäkinen; Michael E Taliansky
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-12-21
Journal Detail:
Title:  Journal of molecular biology     Volume:  357     ISSN:  0022-2836     ISO Abbreviation:  J. Mol. Biol.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-03-13     Completed Date:  2006-04-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985088R     Medline TA:  J Mol Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1-8     Citation Subset:  IM    
Affiliation:
Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK.
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MeSH Terms
Descriptor/Qualifier:
Immunohistochemistry
Microscopy, Atomic Force
Potyvirus / chemistry*,  metabolism
Protein Conformation*
Viral Proteins / chemistry*,  metabolism,  ultrastructure*
Chemical
Reg. No./Substance:
0/Viral Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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